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Improved method for quickly establishing mouse myocardial infarction model

A myocardial infarction and mouse technology, which is applied in the field of rapid establishment of mouse myocardial infarction model improvement, can solve the problems of high operator requirements and difficult operation, achieve high modeling success rate, low operation difficulty, and solve operation difficulty big effect

Pending Publication Date: 2021-07-27
HUBEI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Squeeze out the heart without using mechanical ventilation, and conduct a quick experiment, which is difficult to operate and requires high requirements for the operator

Method used

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  • Improved method for quickly establishing mouse myocardial infarction model
  • Improved method for quickly establishing mouse myocardial infarction model
  • Improved method for quickly establishing mouse myocardial infarction model

Examples

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Effect test

Embodiment 1

[0033] An improved method for rapidly establishing a mouse myocardial infarction model, comprising the steps of: comprising the steps of:

[0034] S1: Prepare experimental animals, prepare n SPF grade Kunming mice, male and female, 6-10 weeks old, weighing 25-35 grams, provided by Liaoning Changsheng Biotechnology Co., Ltd., license: NO.SCXK (Liao) 2020---0001;

[0035] S2: Make a respiratory anesthesia device, connect the oxygen bag and the atomized inhalation drug bottle to make a respiratory anesthesia device, and place the clean cotton ball at the bottom of the atomized drug bottle;

[0036] S3: as attached figure 1 , as shown, remove the mask, stuff the mouse’s head into the mouthpiece of the medicine bottle, control the speed and amount of oxygen entering the medicine bottle by adjusting the valve of the oxygen bag, open the valve of the oxygen bag to the maximum, and then inject the mouse Put the mouthpiece of the isoflurane bottle on the head of the mouse. After 1-2 ...

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Abstract

The invention relates to the technical field of model establishment, and discloses an improved method for quickly establishing a mouse myocardial infarction model. The method comprises the steps that S1 an experimental animal is prepared, namely n SPF (specific pathogen free)-grade Kunming mice which are half in male and female, are 6 to 10 weeks old and have the weight of 25 to 35g are prepared; S2 a respiratory anesthesia device is manufactured, an oxygen bag and an atomization inhalation medicine bottle are connected to be manufactured into the respiratory anesthesia device, and a cleaning cotton ball is placed at the bottle bottom of the atomization medicine bottle; S3 a mask is taken down, the heads of the mice are stuffed into a suction nozzle of the medicine bottle, the speed and the amount of oxygen entering the medicine bottle are controlled by adjusting a valve of the oxygen bag, then 0.5 ml of isoflurane is sucked through a 5 ml injector and added to the cotton ball through a gap in a medicine bottle cover till the cotton ball soaked with the isoflurane volatilizes at the bottom of the medicine bottle and enters the suction nozzle, and meanwhile oxygen is supplied through the oxygen bag until the mice are in a stable anesthetic state. The method disclosed by the invention is simple, is used for quickly anesthetizing the mice and is convenient for subsequent work.

Description

technical field [0001] The invention relates to the technical field of model establishment, in particular to an improved method for rapidly establishing a mouse myocardial infarction model. Background technique [0002] With the development of society and economy, the incidence of cardiovascular diseases is getting higher and higher. Myocardial infarction is a critical and serious disease among cardiovascular diseases. Therefore, it is essential to study the etiology and pathogenesis of myocardial infarction and establish animal models of myocardial infarction. [0003] In order to be closer to the clinic, coronary artery ligation is often used to establish myocardial infarction models at home and abroad. Coronary artery ligation under direct vision is initially suitable for medium and large animals such as rabbits, pigs, and rats, but the cost of experimental animals is high. The experimental cost is reduced but the mortality rate is relatively high when mice are used as a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D1/00
CPCA61D1/00
Inventor 张莉袁林陈世健魏小栋陈功泉王亮
Owner HUBEI UNIV FOR NATITIES
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