Separation method of Colletotrichum musae

A technology of banana anthracnose and anthracnose bacteria, applied in the field of plant pathology, can solve the problems of time-consuming and labor-intensive, affecting the efficiency and effect of separation work, and achieve the effect of improving efficiency and simple separation operation

Active Publication Date: 2021-07-27
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This type of method needs to carry out the spore quantification and dilution operation process of the spore liquid, which is time-consumi

Method used

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  • Separation method of Colletotrichum musae
  • Separation method of Colletotrichum musae
  • Separation method of Colletotrichum musae

Examples

Experimental program
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Example Embodiment

[0035] 2. Preparation of spores

[0036] Remove the gun head of 1000 μL of ordinary pipe gun, put the tip of the gun in the flame, burning, the gun's tip fire is melted, using the alcohol lamp to extinguish the fire cover, etc., the burning fire on the gun, with pointed scorpions Clean a melted gun head, gently elongate, to form a silky filament, cooled to reduce the extra filaments, retain about 3cm long filaments, to form a single Spore discrete wires used by spores used by spores, the discrete wire tool includes a sliding wire a and a slider B, such as figure 1 As shown, these two parts are naturally linked into one, and the key technologies are sliding filaments a. Sliding filament A is small, soft and flexible.

[0037] When separating different specimens, you can use 75% of alcoholic cotton ball to wipe the surface disinfection of the sliding filament A, or repeat the previous operation and firing and stretching a new sliding wire.

[0038] 3. Preparation of spore dispersion...

Example Embodiment

[0058] Example 1

[0059] The separation method of a banana anthracnose is used in the present invention, and the banana anthrax is separated from the banana anthrax. Image 6 Indicated.

Example Embodiment

[0060] Example 2

[0061] The separation method of a banana anthrax is separated from the separation method of the banana anthrax 2, which is separated from the banana anthrax. Figure 7 Indicated.

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Abstract

The invention discloses a method for separating Colletotrichum musae. According to the method, a special spore discrete filament is adopted, and a single conidium is directly separated from a Colletotrichum musae standard sample and is cultured to form a Colletotrichum musae germ strain with a single genetic composition. The implementation operation steps of the isolation culture are as follows: 1) preparation of a Colletotrichum musae standard sample; (2) preparing spore discrete filaments; (3) preparing a spore dispersing plate; (4) dispersing conidia; (5) cutting small square blocks loaded with monospores; and (6) forming a monospore isolated strain. The method has the following advantages: 1) a key appliance for separating spores is simple and easy to manufacture, and the separation operation is simple and easy; 2) single spore strains can be separated and obtained at one time; 3) the separation work consumes less time and labor; and 4) the working efficiency of Colletotrichum musae germ separation and the reliability of the separation result can be obviously improved.

Description

technical field [0001] The present invention relates to phytopathological techniques. Specifically, it is a method for isolating banana anthracnose bacteria. Background technique [0002] Banana anthracnose is a major disease in my country's banana industry, which is caused by the infection of banana anthracnose fungus (Colletotrichum musae). Carrying out biological research on banana anthracnose bacteria is an important basis for the prevention and control of banana anthracnose. The prerequisite for carrying out biological research on banana anthracnose bacteria is to have the materials of banana anthracnose bacteria. General laboratories often need to isolate banana anthracnose bacteria from disease standard samples. [0003] The genetic composition of the banana anthracnose bacterial population is complex, and the biological characteristics and pathogenicity of different strains are often quite different. Normal research usually needs to obtain materials of banana ant...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/02C12N3/00C12R1/645
CPCC12N1/14C12N1/02C12N3/00
Inventor 张容博张君成王忠文
Owner GUANGXI UNIV
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