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Fruit epidermis saccharomycetes separation method, saccharomycetes and application

A separation method, yeast technology, applied in the direction of application, separation of microorganisms, botany equipment and methods, etc., can solve the problems of poor disease resistance, poor biocontrol effect, etc., to reduce secondary damage, strong survivability, and applicable wide range of effects

Pending Publication Date: 2021-07-27
CHONGQING UNIV OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the isolated yeast has defects such as relatively poor biocontrol effect and low disease resistance.

Method used

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  • Fruit epidermis saccharomycetes separation method, saccharomycetes and application
  • Fruit epidermis saccharomycetes separation method, saccharomycetes and application
  • Fruit epidermis saccharomycetes separation method, saccharomycetes and application

Examples

Experimental program
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Effect test

specific Embodiment 1

[0052] A method for isolating fruit epidermis yeast, specifically,

[0053] Step (1) Isolate the yeast from the surface of the plums, wash the fruits and put them directly in a conical flask or beaker with double distilled water, and shake them in a shaker at 25° C. and 200 rpm for 1-2 hours.

[0054] Step (2) Then take 1000 μL of the liquid and inject it into a 1.5ml centrifuge tube, and then carry out concentration gradient dilution. The concentration gradient dilution method in this application is the method commonly used in this field, 10 -1 Dilute sequentially, and take 100 μL of the final diluted solution and spread it on a YPD Petri dish.

[0055] Step (3) After two or three days of culturing in an incubator at 25°C, select a single yeast and put it into a 50ml Erlenmeyer flask containing 20ml of YPD culture solution, and shake overnight at 25°C on a shaker at 200rpm.

[0056] Step (4) Inject 1000 μL of culture solution into a 1.5 ml centrifuge tube and centrifuge in ...

Embodiment 2

[0059] A method for isolating fruit epidermis yeast, similar to Example 1, the difference is that the fruit used in step (1) is a grape, and the yeast isolated from the surface of the grape has a sequence shown in SEQ NO.2 in the sequence table .

Embodiment 3

[0061] A method for isolating fruit epidermis yeast, similar to Example 1, the difference is that the fruit used in step (1) is mangosteen, and the yeast isolated from the surface of mangosteen has a sequence shown in SEQ NO.3 in the sequence table .

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Abstract

The invention provides a fruit epidermis saccharomycetes separation method, saccharomycetes and application, and the method comprises the following steps: (1) putting fruits into a vessel filled with double distilled water, and conducting shaking for a period of time; (2) injecting the liquid obtained in the step (1) into a centrifugal tube, carrying out concentration gradient dilution, and coating a plate with the finally diluted liquid; (3) after culturing in an incubator, selecting single saccharomycetes, putting the single saccharomycetes into a vessel containing a culture solution, and conducting shaking for a period of time; (4) centrifuging the culture solution, pouring out the supernate, injecting double distilled water, uniformly conducting mixing, conducting centrifuging, repeatedly conducting washing, and carrying out concentration gradient dilution; and (5) coating a plate with the finally diluted liquid, conducting culturing in an incubator, and then selecting single saccharomycetes to perform lineation purification in a culture dish. The separation method provided by the invention is simple, efficient and wide in application range, and the separated saccharomycetes also have a biocontrol effect at a relatively low concentration and are of great significance in the aspect of disease control.

Description

technical field [0001] The invention belongs to the field of disease prevention and control, and in particular relates to a method for isolating fruit epidermis yeast, yeast and application thereof. Background technique [0002] At present, the most common fungal diseases of kiwifruit in production practice include penicillium, gray mold, and black spot. In order to reduce the economic loss caused by the postharvest disease of kiwifruit, it is proposed to use biocontrol yeast to control it. At present, post-harvest disease treatment of fruits and vegetables is mainly based on chemical fungicides. The defect is that chemical residues will cause major damage to the environment, and at the same time, it is easy to cause poisoning of humans and animals, kill natural enemies, and cause secondary pests to rampant again. Pesticides can cause some pests to produce different degrees of resistance and other disadvantages. [0003] Compared with chemical control, biological control h...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N1/02A01N63/32A01P3/00
CPCC12N1/16C12N1/02A01N63/32
Inventor 隋媛刘嘉冷进松
Owner CHONGQING UNIV OF ARTS & SCI
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