Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for extracting astragalus membranaceus lectin protein from astragalus membranaceus seeds

A technology of Astragalus membranaceus and lectin, which is applied in the biological field, can solve the problem of low extraction rate of Astragalus membranaceus seeds, and achieve the effect of increasing protein extraction rate and extraction amount

Pending Publication Date: 2021-07-30
江苏朴荷生物科技有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a method for extracting the lectin protein of Astragalus membranaceus by using the seeds of Astragalus membranaceus, which solves the problem of low extraction rate of Astragalus membranaceus seeds in the existing extraction method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The embodiment of the present invention provides a method for extracting the lectin protein of Astragalus membranaceus by using the seeds of Astragalus membranaceus, using the seeds of Astragalus membranaceus as the raw material, comprising the following steps:

[0030] S1. Preprocessing:

[0031] Rinse the raw materials with clean water at 25°C for 12 minutes, then shell and crush the seeds, soak them in warm water at 4°C for 1.5 days, and then stir them into a slurry;

[0032] S2, extraction:

[0033] Filter water through a 40um microporous membrane, then add agar powder with a volume of 2% of the slurry to the slurry, and stir evenly with the slurry, then place it in a centrifuge, centrifuge at 18000r / min for 20min, collect the supernatant, and then add 30ml of 20mmol / L, pH7.0 PBS buffer solution was dissolved, put into dialysis bags for dialysis, and the initial product was obtained after repeated dialysis;

[0034] S3, purification:

[0035] Separate and purify ...

Embodiment 2

[0045] The embodiment of the present invention provides a method for extracting the lectin protein of Astragalus membranaceus by using the seeds of Astragalus membranaceus, using the seeds of Astragalus membranaceus as the raw material, comprising the following steps:

[0046] S1. Preprocessing:

[0047] Rinse the raw materials with clean water at 35°C for 25 minutes, then shell and crush the seeds, soak them in warm water at 55°C for 3 days, and then stir them into a slurry;

[0048] S2, extraction:

[0049] Filter the water through a 40um microporous membrane, then add agar powder with a volume of 5% of the slurry to the slurry, and stir evenly with the slurry, then place it in a centrifuge, centrifuge at 18000r / min for 20min, collect the supernatant, and then add 30ml of 20mmol / L, pH7.0 PBS buffer solution was dissolved, put into dialysis bags for dialysis, and the initial product was obtained after repeated dialysis;

[0050] S3, purification:

[0051] Separate and puri...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for extracting astragalus membranaceus lectin protein from astragalus membranaceus seeds, and relates to the technical field of biology. The method for extracting the astragalus membranacus lectin protein from the astragalus membranacus seeds takes the astragalus membranacus seeds as a raw material, and comprises the following steps: S1, pretreatment: firstly washing the raw material with clear water at 25-35 DEG C, then shelling and grinding the seeds, soaking the seeds in warm water at 40-55 DEG C for 1.5-3 days, and then stirring the seeds into slurry; S2, extraction: filtering water through a 40 [mu] m microfiltration membrane, then adding agar powder which is 2-5% of the volume of the slurry into the slurry, uniformly stirring the agar powder and the slurry, then putting the mixture into a centrifugal machine for centrifugation, collecting supernate, putting the supernate into a dialysis bag for dialysis, and obtaining an initial product after multiple times of dialysis. Pretreatment is carried out before extraction, sufficient and uniform slurry is prepared, then extraction and purification are carried out, agar powder is added, part of protein can be combined with the agar powder, and most lectin protein is reserved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting astragalus membranaceus lectin protein from astragalus membranaceus seeds. Background technique [0002] Astragalus membranaceus is a kind of traditional Chinese medicine, which has the functions of invigorating qi and raising yang, benefiting health and strengthening the surface, diuresis and swelling, and treating sores and promoting granulation. It has extremely high medicinal value and has been planted and cultivated on a large scale in my country. Lectins have a variety of biological functions, and their potential research value has been gradually excavated. Lectins are a class of non-immune proteins or glycoproteins that can reversibly and specifically interact with carbohydrates, have variable amino acid sequences, and are widely distributed in microorganisms, viruses and plants. Lectins have multiple functions, structures, tissue localizat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/42C07K1/36C07K1/34C07K1/16
CPCC07K14/42
Inventor 张晓秋
Owner 江苏朴荷生物科技有限公司