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Capsicum-tolerant ethanol high-yield yeast as well as screening method and verification method thereof

A verification method, yeast technology, applied in the field of waste resource recycling, can solve the problems of microbial growth and fermentation inhibition, reduce the utilization effect of food waste, complex food waste components, etc., and achieve high efficiency

Pending Publication Date: 2021-07-30
GUANGDONG QIZHI BIOTECHNOLOGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are still defects in the existing microorganisms used in the degradation of food waste. The components of food waste are relatively complex. Capsicum is a component that often appears in food waste, and capsaicin, the active ingredient of chili, will affect the microorganisms. Growth and fermentation are affected. In the presence of a certain amount of capsaicin, the growth and fermentation of microorganisms will be significantly inhibited, which hinders the direct fermentation of food waste by using microorganisms and reduces the utilization effect of food waste.

Method used

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  • Capsicum-tolerant ethanol high-yield yeast as well as screening method and verification method thereof
  • Capsicum-tolerant ethanol high-yield yeast as well as screening method and verification method thereof
  • Capsicum-tolerant ethanol high-yield yeast as well as screening method and verification method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0043] A screening method for ethanol high-yielding yeast tolerant to capsicum, comprising the following steps:

[0044] S1, screening out the high-yielding yeast strain of ethanol in sugarcane molasses, denoted as LSK1;

[0045] Specifically, in this example, 5 g of sugarcane molasses was added to YPD liquid medium containing 2% glucose, and after shaking overnight for 24 hours, 1 mL of culture solution was taken out and diluted with sterile water for 10 -6 10 -8 times, respectively spread on the YPD solid medium plate containing 2% glucose, and cultivate for 12 hours. After a single colony grows, pick it into the liquid medium and cultivate it for 12 hours. Take a small amount of bacterial liquid and observe the colony shape under a microscope to determine the yeast strain. Afterwards, the ability of each yeast strain to ferment and utilize glucose was measured, and the strain with stronger ethanol production ability was selected and named as LSK1 strain.

[0046] S2. Make...

Embodiment 2

[0054] A verification method for ethanol-producing yeast tolerant to capsicum, comprising the following steps:

[0055] S1, the ethanol high-yielding yeast strain in the sugarcane molasses is screened out, denoted as LSK1;

[0056] S2. Make the LSK1 strain undergo adaptive evolution of pepper tolerance, and obtain the adapted strain, which is denoted as LSK1pe;

[0057] S3. Construct an environment where capsaicin exists, and verify the ability of the LSK1pe strain to tolerate capsicum based on the homologous strain LSK1.

[0058] Wherein, S1 and S2 in this embodiment are the same as those in Embodiment 1, and will not be repeated here. Step S3 constructs the environment where capsaicin exists, and verifies the ability of the LSK1pe strain to tolerate capsicum based on the homologous strain LSK1, including comparing the growth status of LSK1 and LSK1pe under the capsicum environment, the ability to use glucose fermentation, and the ability to use starch fermentation to verify...

Embodiment 3

[0069] A high-yielding yeast tolerant to capsicum, the strain obtained by screening the screening method in Example 1 or verifying the verification method in Example 2 is based on the high-yielding strain selected from sugarcane molasses and obtained through the adaptive evolution of capsicum tolerance , at least it can be used for microbial fermentation of food waste containing capsaicin, or as a basic strain for constructing better strains.

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Abstract

The invention relates to a screening and verification method of capsicum-tolerant ethanol high-yield yeast, and the screening method comprises the following steps: S1, screening out an ethanol high-yield yeast strain from cane molasses, and recording the ethanol high-yield yeast strain as LSK1; and S2, carrying out adaptive evolution of pepper tolerance on the LSK1 strain to obtain an adapted strain, and recording the adapted strain as LSK1pe. The verification method comprises the following steps on the basis of the screening method: step S3, constructing an environment in which capsaicin exists, and verifying the pepper tolerance of the LSK1pe strain based on the homologous strain LSK1. According to the screening method, the ethanol high-yield yeast evolved through pepper adaptability can be obtained, and the yeast obtained through the screening method can be applied to microbial fermentation of kitchen waste containing pepper. The verification method can be used for further carrying out pepper tolerance verification on the pepper tolerance and ethanol high-yield yeast obtained by screening, so that the verified and confirmed yeast can be obtained, and the subsequent practical application is facilitated.

Description

technical field [0001] The invention relates to the field of regeneration and utilization of waste resources, and more particularly relates to a capsicum-tolerant ethanol-producing yeast and a screening method and verification method thereof. Background technique [0002] Kitchen waste, commonly known as swill, is solid waste formed by residents in their daily lives. The amount of food waste is increasing rapidly with the development of economy and society. The main components of food waste include meat, vegetables, oil, rice and other substances. Due to the huge output of food waste, Staphylococcus aureus, Salmonella and other toxic and harmful pathogenic microorganisms often Breeding in garbage, if it is not treated in time, it will be accompanied by a large amount of odorous gas NH 3 and H 2 Production of substances such as S and toxins. Not only will it cause serious environmental problems, but it will also cause harm to human health and harm. Therefore, it is necess...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12P7/08C12R1/645
CPCC12Q1/04C12P7/08
Inventor 刘泽寰林蒋海孙涛
Owner GUANGDONG QIZHI BIOTECHNOLOGY CO LTD
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