Biosensor for ctDNA detection and preparation method thereof

Abstract

The invention provides a preparation method of a biosensor for ctDNA detection, which comprises the following steps: preparing gold nanoparticles: mixing and heating chloroauric acid and ultrapure water, then adding a sodium citrate solution, continuously conducting heating and reacting, conducting cooling to room temperature after the reaction is finished, conducting filtering to obtain the gold nanoparticles, and conducting preserving at low temperature for later use; and conducting DNA modification: carrying out annealing treatment on DNA, then uniformly mixing the DNA subjected to annealing treatment with the prepared gold nanoparticles, carrying out standing treatment, then adding a sodium citrate solution, then adding a phosphate buffer solution, carrying out standing, finally adding water, and carrying out centrifugal washing, thus obtaining the biosensor for ctDNA detection.

Description

technical field

[0001] The invention relates to the technical field of DNA tumor analysis, in particular to a biosensor for ctDNA detection and a preparation method thereof. Background technique

[0002] Tumor is a serious disease that threatens human health. Currently, the main body of cancer diagnosis, staging, and treatment decisions is tissue biopsy. Although tissue biopsy is very useful, the invasiveness and inherent selection preference of biopsy limit its usefulness as a real-time monitoring tool, making it difficult to use for early diagnosis, and Invasive tissue biopsies often cause great pain to patients. In order to overcome this shortcoming, people began to look for various tumor biomarkers, such as protein markers, circulating tumor cells and circulating tumor DNA (circulating tumor DNA, ctDNA) and so on. In recent years, studies have shown that the tumor mutation information carried by ctDNA of tumor patients has a good consistency with tumor tissue. Therefo...

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