Kit for quantitatively detecting serum amyloid protein A and preparation method thereof

Abstract

The invention discloses a kit for quantitatively detecting human serum amyloid protein A. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises 50 to 200mM of a buffer solution, 0.1 to 0.5% of a surfactant I, 0.1 to 0.5% of a surfactant II, 0.1 to 0.5% of a preservative, 0.1 to 0.8% of inorganic salt and 1 to 2% of a coagulant; and the reagent R2 comprises 50 to 100mM of a buffer solution, 1 to 5% of a protein protective agent, 0.1 to 0.5% of a preservative, 0.1 to 0.8% of inorganic salt and latex particles coated with an SAA monoclonal antibody. According to the invention, a benzoic acid and morpholine composition is added during the labeling process of the latex microspheres, such that the labeling efficiency of the latex microspheres and the antibody is increased, the HOOK resistance of the reagents during the detection of a high-value positive sample is significantly improved, and the linear range of the reagents can achieve 2 to 500 mg / L.

Description

technical field [0001] The invention relates to the field of medical testing, in particular to a kit for quantitatively detecting human serum amyloid A and a preparation method thereof. Background technique [0002] Serum amyloid A (serum amyloid Aprotein, SAA) is an acute phase reaction protein produced by hepatocytes and then secreted into serum. Lipoprotein (HDL) binding. The content of SAA in patients with acute phase of inflammation or infection increases rapidly within 48-72 hours, and rapidly decreases during the recovery phase of the disease. As a new detection index for acute phase of inflammation or infection, SAA plays an important role in clinical application. [0003] In a normal human body, the content of SAA is below 10 mg / L. When a slight infection occurs, the content of SAA in the human body rises and is within the range of 10-200 mg / L. However, for patients with some diseases, the content of SAA in their body will increase significantly. For example, the ...

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Problems solved by technology

[0004] At present, the detection methods of SAA include ELISA and latex-enhanced immunoturbidimetric method, etc. ELISA is not conducive to high-throughput detection due to its cumbersome process and long detection time.

The latex-enhanced immunoturbidimetric method has the advantages of fast detection speed and high accuracy. It ensures the accuracy of detection while achieving high-throughput detection. It has gradually developed into an important clinical detection method. However, the SAA latex currently on the market Enhanced immunoturbidimetric detection kits have shortcomings such as narrow detection linearity (most reagents have a linear range below 200mg / L) and weak anti-HOOK ability, which is not conducive to the diagnosis of diseases and the judgment of different medical levels

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