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Method for stimulating indigenous flora to rapidly degrade cypermethrin in soil

A rapid degradation, cypermethrin technology, applied in the field of bioremediation of pesticide-contaminated soil, can solve problems such as acceleration, achieve the effect of improving soil quality, improving the ability to resist pesticide poisoning, and improving the degradation of soil pesticides

Active Publication Date: 2021-08-10
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Technical problem to be solved: The present invention provides a method for stimulating the rapid degradation of cypermethrin in soil by indigenous flora, which can effectively accelerate the degradation of cypermethrin by indigenous flora, reduce the use and repair cost of soil pollution remediation materials, and reduce the impact of remediation reagents and catalysts on Soil environment disturbance, improve soil quality and ecological environment self-recovery ability

Method used

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  • Method for stimulating indigenous flora to rapidly degrade cypermethrin in soil
  • Method for stimulating indigenous flora to rapidly degrade cypermethrin in soil
  • Method for stimulating indigenous flora to rapidly degrade cypermethrin in soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Structural composition analysis of domesticated target flora in earthworm intestinal reactor in soil contaminated with different concentrations of cypermethrin

[0021] Select healthy adult C. williamicae with a body weight of 3-5 g, wash them with sterile water, and put them in a dark box in a dark box for 12 h of intestinal cleansing, and the earthworms after complete intestinal cleansing are washed again spare. Mix the purchased cypermethrin into an appropriate amount of diatomaceous earth, and add clean soil to prepare cypermethrin-contaminated soil with a concentration of 0.5, 5, and 20 mg / kg. Referring to one earthworm per 50 g of soil, three 50 kg concentrations of 0.5, 5, and 20 mg / kg cypermethrin-contaminated soil experimental groups were set up. The experiment was carried out in a dark greenhouse, the indoor temperature was kept at about 25°C, and the domestication experiment was carried out for 20 days. After domestication, earthworms in different...

Embodiment 2

[0023] Example 2 Targeted flora, indigenous flora and targeted flora-indigenous flora remediation of soil contaminated with cypermethrin at different concentrations

[0024] Select healthy adult C. williamicae with a body weight of 3-5 g, wash them with sterile water, and put them in a dark box in a dark box for 12 h of intestinal cleansing, and the earthworms after complete intestinal cleansing are washed again spare. Mix the purchased analytically pure standard cypermethrin into an appropriate amount of diatomaceous earth, and add clean soil to prepare cypermethrin-contaminated soil with a concentration of 0.5, 5, and 20 mg / kg. Referring to one earthworm per 50 g of soil, three 50 kg concentrations of 0.5, 5, and 20 mg / kg cypermethrin-contaminated soil experimental groups were set up. The experiment was carried out in a dark greenhouse, the indoor temperature was kept at about 25°C, and the domestication experiment was carried out for 20 days. After domestication, earthwor...

Embodiment 3

[0027] Example 3 Effect of soil culture cycle on targeted flora-indigenous flora to remediate soil contaminated with cypermethrin at different concentrations

[0028] Select healthy adult C. williamicae with a body weight of 3-5 g, wash them with sterile water, and put them in a dark box in a dark box for 12 h of intestinal cleansing, and the earthworms after complete intestinal cleansing are washed again spare. Mix the purchased analytically pure standard cypermethrin into an appropriate amount of diatomaceous earth, and add clean soil to prepare cypermethrin-contaminated soil with a concentration of 0.5, 5, and 20 mg / kg. Referring to one earthworm per 50 g of soil, three 50 kg concentrations of 0.5, 5, and 20 mg / kg cypermethrin-contaminated soil experimental groups were set up. The experiment was carried out in a dark greenhouse, the indoor temperature was kept at about 25°C, and the domestication experiment was carried out for 20 days. After domestication, earthworms in d...

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Abstract

The invention discloses a method for stimulating indigenous flora to quickly degrade cypermethrin in soil, and the method comprises the following steps: selecting living adult earthworms domesticated in clean soil, cleaning the living adult earthworms, and placing the living adult earthworms in a dark box for gut purging; putting the earthworms subjected to intestine cleaning treatment into cypermethrin polluted soil with the preparation concentration of 0.5-20 mg / kg, and performing domesticating for 20 days; taking out the cultured earthworms, performing cleaning, dissecting intestinal tracts, removing contents in the intestinal tracts, and scraping intestinal wall flora; according to the mass ratio of the put earthworm intestinal flora to the soil being 1: 2000-1: 8000, adding the earthworm intestinal flora into the soil to be remediated, and performing remediation for 7-60 days. Under the stress of cypermethrin, the targeted flora domesticated by an earthworm intestinal reactor is rich in cypermethrin pesticide degrading microorganisms, and when the targeted flora is added into the cypermethrin polluted soil, the indigenous flora is promoted to quickly degrade the cypermethrin, the degradation rate can reach 96.1%, and the ecological environment safety of the soil is further maintained.

Description

technical field [0001] The invention belongs to the technical field of bioremediation of pesticide-contaminated soil, and in particular relates to a method for domesticating and targeting flora in an earthworm intestinal reactor to stimulate native flora to rapidly degrade cypermethrin in soil. Background technique [0002] As a big country in the production and use of pesticides, my country’s pesticide output reached 3.78 million tons in 2016, accounting for more than 1 / 3 of the world’s total. In 2018, the amount of pesticides used reached 1.504 million tons. These pesticides have caused serious soil pollution during production, transportation and use. question. As one of the most common pesticides in modern times, cypermethrin is ubiquitous in the soil environment and has a high detection rate. The main non-point source of pollution is agricultural soil. Cypermethrin is mainly used to kill pests in farmland, cultivated land, woodland and other soils. Due to large and exc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
CPCB09C1/10
Inventor 叶茂朱国繁卞永荣蒋新
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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