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Siderophore-producing Aspergillus centimensis and its application in the control of plant diseases

A technology for plant disease and centipede, which is applied in the field of Aspergillus centipede and its application field of preventing and controlling plant diseases, and can solve the problems of insufficient microbial resource excavation and the like

Active Publication Date: 2022-04-01
INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the problem of insufficient exploration of siderophore microbial resources in nature
As for Aspergillus welwitschiae, more studies have focused on the application of its metabolites in regulating the microbial community structure, and there are few reports on siderophores.

Method used

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  • Siderophore-producing Aspergillus centimensis and its application in the control of plant diseases
  • Siderophore-producing Aspergillus centimensis and its application in the control of plant diseases

Examples

Experimental program
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preparation example Construction

[0018] The method for preparing carboxylate-type siderophore provided by the present invention comprises: inoculating the Aspergillus centifolia of the present invention into a (liquid) medium for culturing. The (liquid) medium may contain: glucose 25-30g / L, sodium nitrate 1-2g / L, potassium phosphate trihydrate 1-2g / L, potassium chloride 0.3-0.8g / L, magnesium sulfate heptahydrate 0.3 -0.8g / L, 8-hydroxyquinoline 0.5-1g / L, pH6.8-7. The cultivation conditions may include: the temperature is 20-30° C., and the time is 48-96 hours.

[0019] According to the present invention, the method may further include performing an extraction operation on the culture product to obtain carboxylate-type siderophore therefrom. The method of extraction operation may be methanol leaching, that is, soaking the culture product with methanol. The amount of methanol used may be 0.5-1 L per liter of culture product. The temperature of extraction can be 25-30°C, and the time can be 24-48h.

[0020] A...

Embodiment approach

[0028] According to another preferred embodiment of the present invention, the culture is carried out in a steamed rice culture medium, and the culture method is 30-60 days at 25-30° C. under dark conditions. The method may also include extracting the culture product to obtain carboxylate siderophores and antibacterial active substances therefrom. The method of extraction operation may be methanol leaching, that is, soaking the culture product with methanol. The amount of methanol used may be 0.25-0.5 L per gram of the culture product. The temperature of extraction can be 25-37°C, and the time can be 24-48h.

[0029] According to the present invention, the plants can be various crops, preferably plants susceptible to bacterial wilt, and more preferably, the plants are selected from tomato.

[0030] According to the present invention, the metabolites of the bacterial strain of the present invention are more outstanding to the pathogenic bacteriostatic activity of tomato bacte...

Embodiment 1

[0037] This example can illustrate the results of morphological identification and molecular genetic classification identification of the strains involved in the present invention.

[0038] A fungus was isolated from the virgin forest soil (100°49′-101°53′E, 23°95′-24°17′N, 2600m above sea level) of Ailaoshan National Nature Reserve, Yunnan Province, China, and the number is 1-5F1, which was deposited in the China Center for Type Culture Collection with the accession number CCTCC NO: M 2021249.

[0039] It is cultured on plate medium (300g of potatoes, 30g of glucose, 30g of agar, 1.5L of deionized water, pH7), and the colony form is round, brownish yellow, opaque, and there are fluffy hyphae on the surface of the colony; scanning After electron microscope magnification 20000 times, the conidia of the bacterial strain are tightly spaced and round, with a diameter of 3.5-4 μm, and the surface of the conidia is smooth. On the basis of morphological observation, the ITS sequenci...

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Abstract

The invention relates to the field of agricultural microorganisms, and discloses a siderophore-producing Aspergillus centenarian and its application in preventing and controlling plant diseases. The preservation number of Aspergillus centenarians disclosed in the present invention is CCTCC NO: M 2021249. In addition, the present invention also provides the application of said Aspergillus centifolia in preparing carboxylate siderophores and regulating plant diseases. The aspergillus centenarian of the invention is a dominant strain of high-yield siderophore, which can synthesize and secrete carboxylate type siderophore. The secondary metabolites of Aspergillus centenarians of the present invention can improve the activity value of invertase and urease, the AWCD value, the functional diversity and richness of microorganisms in tomato bacterial wilt diseased soil.

Description

technical field [0001] The invention relates to the field of agricultural microorganisms, in particular to Aspergillus centenarians with siderophore activity and its application in preventing and controlling plant diseases. Background technique [0002] Iron plays an important role in the process of microbial growth, development and metabolism (for example, it is essential for the formation of biofilm. Under the growth conditions of iron deficiency, the hydrophobicity of the microbial surface is reduced, resulting in the formation of biofilm is limited), while In nature, it mainly exists in the stable form of insoluble trivalent state. Microorganisms usually secrete ferric ions (Fe 3+ ) siderophore with chelating ability to compete for free iron in the environment. while for Fe 3+ Siderophores with high affinity are a class of small molecular compounds produced by most microorganisms, and the main types include catechols, hydroxamates and carboxylates. [0003] Sideropho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/34A01P1/00A01P21/00C12R1/66
CPCC12N1/14A01N63/34
Inventor 杨佩文李铭刚邹雪峰赵江源何翔张庆杨济达朱红业杨明英
Owner INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI