Filamentous pseudomonas strain for degrading sulfamethoxazole and application thereof
A technology of sulfamethoxazole and Pseudomonas, applied in the field of microorganisms, can solve the problems of less research on microbial degradation of proteins and molecular mechanisms, unfavorable understanding of SMX degradation, and difficulty in understanding the metabolic process of SMX, and achieves strong sulfamethoxazole. Effect of oxazole degradability
Active Publication Date: 2021-08-10
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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Problems solved by technology
Bioelectrochemical technology mainly improves the removal effect of SMX by strengthening the regulation of microorganisms in constructed wetlands. However, bioelectrochemical enhanced constructed wetlands are a relatively complex system, and it is difficult to understand the metabolic process of SMX in it, which is not conducive to the construction of constructed wetlands. Further understanding of the degradation process of SMX
At present, the research on SMX-degrading bacteria in constructed wetlands is very limited, and the research that has been carried out is mainly around the screening of SMX-degrading bacteria in activated sludge or sediment contaminated by antibiotics, intermediate products, degradation kinetics, and co-metabolism analysis. However, there are few studies on the protein and molecular mechanism of microbial degradation
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[0054] 1. Test materials and methods
[0055] 1.1 Screening and isolation of SMX degrading bacteria
[0056] (1) Experiment medium. According to the experimental requirements, the R2A medium and minimal mineral salt medium (MSM) of the strains are shown in Table 1. Adjust the pH to 7.0~7.2, and add 15 g·L of solid medium -1 Agar, all media were autoclaved at 121°C for 20 minutes (after cooling to 55-60°C, 50% sterile glucose was added) for later use.
[0057] (2) Enrichment, isolation and purification of SMX degrading bacteria. The anode and anode matrix samples of DC-enhanced constructed wetland (EC-CW) and microbial fuel cell-enhanced constructed wetland (MFC-CW) devices were collected respectively. Rinse each sample with sterile water, wash down the microorganisms in the sample by vortex shaking, and shake well. Under the condition of energization (0.4 V), in the ultra-clean workbench (anaerobic aseptic operation bench-system lower sample and vertical wind ultra-clean ...
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The invention relates to the field of microorganisms, in particular to a filamentous pseudomonas strain for degrading sulfamethoxazole and application of the filamentous pseudomonas strain. The strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC No: M2021338; the preservation time is April 6, 2021. The strain has strong sulfamethoxazole degradation capability, and can realize removal of more than 75% of SMX in an R2A culture medium; meanwhile, the strain is also an electrochemical active bacterium.
Description
technical field [0001] The invention relates to the field of microorganisms, in particular to a filamentous Pseudomonas strain degrading sulfamethoxazole and an application thereof. Background technique [0002] Sulfamethoxazole (SMX), as a typical class of sulfonamide antibiotics, can interrupt bacterial synthesis, thereby inhibiting bacterial growth. Bioelectrochemical technology mainly improves the removal effect of SMX by strengthening the regulation of microorganisms in constructed wetlands. However, bioelectrochemical enhanced constructed wetlands are a relatively complex system, and it is difficult to understand the metabolic process of SMX in it, which is not conducive to the construction of constructed wetlands. Further understanding of the process of degrading SMX. At present, the research on SMX-degrading bacteria in constructed wetlands is very limited, and the research that has been carried out is mainly around the screening of SMX-degrading bacteria in activat...
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IPC IPC(8): C12N1/20C02F3/34C02F11/02B09C1/10C12R1/38C02F101/38
CPCC12N1/20C02F3/34C02F11/02B09C1/10C02F2101/40Y02W10/10Y02E60/50C12N1/205C02F3/341C12R2001/01C12R2001/38
Inventor 刘晓晖卢少勇陈静徐佳敏王晶邱东茹王永强国晓春刘莹
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI