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Method for detecting main medicinal components of cinnamon and storax in medicinal preparation

A technology for pharmaceutical preparations and active ingredients, applied in the detection field of cinnamic acid, can solve problems such as lack, and achieve the effects of ensuring stability, good stability, and improving quality control system

Pending Publication Date: 2021-08-10
HEHUANG PHARMA SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the shortcomings of the prior art described above, the purpose of the present invention is to provide a detection method for the main medicinal components of cinnamon and styrax in pharmaceutical preparations, which is used to solve the lack of common ingredients of cinnamon and styrax in pharmaceutical preparations in the prior art. The main medicinal ingredients: the problem of the content determination method of cinnamic acid

Method used

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  • Method for detecting main medicinal components of cinnamon and storax in medicinal preparation
  • Method for detecting main medicinal components of cinnamon and storax in medicinal preparation
  • Method for detecting main medicinal components of cinnamon and storax in medicinal preparation

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preparation example Construction

[0056] 1. Preparation of the test solution

[0057] Grind the pharmaceutical preparation sample into powder, add organic solvent to dissolve, and accurately weigh. Wherein, the ratio of the mass of the pharmaceutical preparation sample added to the volume of the organic solvent added is 1:20-50, g / mL. Ultrasonic extraction was then performed for 10-40 min at a power of 250-350 W and a frequency of 35-45 kHz. Cool, weigh again, and make up for the weight loss with an organic solvent. Shake well, take the supernatant and pass it through a 0.45μm filter membrane, discard the initial filtrate, and obtain the subsequent filtrate, which is the solution of the test product. The above organic solvent is selected from one of methanol, ethanol, acetonitrile, 95% ethanol, and 80% methanol.

[0058] 2. Preparation of reference solution

[0059] The cinnamic acid reference substance was added into an organic solvent to dissolve and constant volume to prepare a reference substance solut...

Embodiment 1

[0071] 1. Preparation of the test solution

[0072] Grind 120 pills of the pharmaceutical preparation sample into powder, take 1.0 g and accurately weigh it, put it in a stoppered Erlenmeyer flask, accurately add 20 ml of 80% methanol to dissolve, seal it tightly, and accurately weigh it. Ultrasonic extraction was then performed for 20 min at a power of 300 W and a frequency of 40 kHz. Cool, weigh again, and make up for the weight loss with 80% methanol. Shake well, take the supernatant and pass through a 0.45 μm filter membrane, and discard the initial filtrate to obtain the subsequent filtrate, which is the test solution 1#.

[0073] 2. Preparation of reference solution

[0074] The cinnamic acid reference substance was dissolved in methanol and fixed to volume to prepare the reference substance solution 1#. The content range of cinnamic acid in reference substance solution 1# is 5.34-222.40 μ g / ml.

[0075] 3. Determination

[0076] Use high performance liquid chromato...

Embodiment 2

[0085] Get the pharmaceutical preparation sample of batch number H160350, adopt the preparation need testing solution in the step 1 in the embodiment 1. At the same time, the reference substance solution was prepared according to step 2 in Example 1, wherein the content of cinnamic acid in the reference substance solution was 60 μg / ml. Accurately draw reference substance solution and need testing solution 10 μ l respectively, inject liquid chromatograph, measure by step 2 in embodiment 1, compare retention time and carry out qualitative, specifically see Fig. 1, determine the output of cinnamic acid in need testing solution For peak conditions, see Table 2 for specific data.

[0086] Table 2

[0087]

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Abstract

The invention provides a method for detecting main medicinal components of cinnamon and storax in a medicinal preparation. The method comprises the following steps: dissolving a medicinal preparation sample in an organic solvent, carrying out ultrasonic extraction, cooling, uniformly shaking, filtering, taking a test solution obtained by subsequent filtrate, detecting by high performance liquid chromatography (HPLC), and determining the content of cinnamic acid which is a main medicinal component shared by the cinnamon and the storax in the test solution. The method for detecting the main medicinal components of the cinnamon and the storax in the medicinal preparation, provided by the invention, is good in precision, reproducibility and stability, accurate and reliable, can truly reflect the quality difference of the cinnamon and the storax in the medicinal preparation, and comprehensively improves a quality control system of the medicinal preparation.

Description

technical field [0001] The invention belongs to the technical field of detection of traditional Chinese medicine components, and relates to a method for detecting the main medicinal components of cinnamon and styrax in pharmaceutical preparations, in particular to a detection method for the main medicinal components shared by cinnamon and styrax in pharmaceutical preparations: cinnamic acid. Background technique [0002] The drug preparation was improved from the Suhexiang pill in the Song Dynasty in the 1970s. It has become one of the most commonly used drugs for the treatment of coronary heart disease because of its curative effect, safety and economy. It relieves symptoms quickly and has no contraindications to nitrates. long-term use can protect blood vessels, prevent atherosclerosis, reduce the number of angina pectoris attacks, and reduce the occurrence of severe cardiovascular events. Chest obstruction caused by chest pain, symptoms of precordial pain, immobilization;...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/74G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/74G01N30/8675G01N2030/047
Inventor 顾佳敏姜鹏詹常森温方方孔瑶田禾苗
Owner HEHUANG PHARMA SHANGHAI