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Non-diagnostic method for preliminarily inferring cause of death based on endogenous metabolite and method for detecting endogenous metabolite

A metabolite and endogenous technology, applied in the field of forensic science, can solve problems such as difficult to accurately determine the cause of death

Active Publication Date: 2021-08-10
HEBEI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In view of the technical problem in the prior art that it is difficult to accurately determine the cause of death in cases lacking other characteristic morphological features, the present invention provides a method for preliminarily inferring the cause of death based on endogenous metabolites for non-diagnostic purposes

Method used

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  • Non-diagnostic method for preliminarily inferring cause of death based on endogenous metabolite and method for detecting endogenous metabolite
  • Non-diagnostic method for preliminarily inferring cause of death based on endogenous metabolite and method for detecting endogenous metabolite
  • Non-diagnostic method for preliminarily inferring cause of death based on endogenous metabolite and method for detecting endogenous metabolite

Examples

Experimental program
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Effect test

Embodiment 1

[0061] The embodiment of the present invention provides a method for detecting endogenous metabolites acetylcarnitine, propionylcarnitine and succinic acid, the specific operation is as follows:

[0062] 1.1 Preparation of test solution

[0063] Take 1.5mL of blood to be tested with anticoagulant in EP tube, centrifuge at 5000r / min at 4°C for 5min to obtain the first supernatant; centrifuge the first supernatant at 12000r / min for 10min to obtain the second supernatant solution; add chromatographically pure methanol equivalent to 3 times its volume to the second supernatant, mix evenly, sonicate for 10 minutes, let stand at -20°C for 15 minutes, and then centrifuge at 12000r / min for 10 minutes to obtain the third supernatant; The third supernatant was dried by nitrogen blowing, then reconstituted by adding ultrapure water (miliQ water) equivalent to 5 times the volume of the third supernatant, filtered, and used as the test solution for inspection.

[0064] 1.2 Sample detectio...

Embodiment 2

[0092] This example provides a method for preliminarily deducing the cause of death of a mouse death sample based on endogenous metabolites. The specific operation steps are:

[0093] 2.1 Preparation of blood samples

[0094] Collect blood samples from mice poisoned to death by chlorpromazine, perphenazine, olanzapine, and clozapine, and prepare poisoned and deadly test solution according to the operation process of 1.1 in Example 1;

[0095] Collect blood samples from mice killed by drowning, suffocation, hemorrhagic shock, and severed cervical cord, and prepare non-poisoned lethal test solution according to the operation process of 1.1 in Example 1;

[0096] 2.2 Detection of blood samples

[0097] Detect respectively the content of acetylcarnitine, propionylcarnitine and succinic acid in poisoned and lethal test solution and non-poisoned lethal test solution with ultra-high performance liquid chromatography-mass spectrometry, and the chromatographic conditions are the same...

Embodiment 3

[0108]This example provides a method for forensic medicine to preliminarily deduce the cause of death of a human death sample based on endogenous metabolites. The specific operation steps are:

[0109] 3.1 Preparation of blood samples

[0110] Collect the blood samples of death cases caused by poisoning by chlorpromazine, perphenazine, olanzapine, and clozapine, and prepare the test solution for death by poisoning according to the operation process of 1.1 in Example 1;

[0111] Collect non-drug-lethal blood samples, and prepare non-poisoning lethal test solution according to the operation process of 1.1 in Example 1;

[0112] 3.2 Detection of blood samples

[0113] Detect respectively the content of acetylcarnitine, propionylcarnitine and succinic acid in poisoned and lethal test solution and non-poisoned lethal test solution with ultra-high performance liquid chromatography-mass spectrometry, and the chromatographic conditions are the same as 1.2. 1. The mass spectrometry ...

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Abstract

The invention belongs to the technical field of forensic medicine, and particularly relates to a method for preliminarily deducing a dead cause based on endogenous metabolites. According to the method, acetylcarnitine, propionyl carnitine and succinic acid in a blood sample are taken as differential metabolites, preliminary deduction of lethal reasons of a main body of an unknown blood sample is realized by combining quantitative analysis with mathematical modeling, and the method can be used for preliminarily judging whether the lethal factors of a death case are poisoning caused by excessive use of antipsychotic drugs or not; and the related antipsychotic drug comprises one or more of chlorpromazine, perphenazine, olanzapine and clozapine. The invention further relates to a method for detecting the endogenous metabolites. The method is used for detecting the endogenous metabolites generated after chlorpromazine, perphenazine, olanzapine and clozapine are taken in a blood sample.

Description

technical field [0001] The invention belongs to the technical field of forensic medicine, and in particular relates to a method for preliminarily estimating the cause of death based on endogenous metabolites for non-diagnostic purposes and a method for detecting endogenous metabolites. Background technique [0002] Chlorpromazine is a traditional typical antipsychotic, clinically mainly used for the treatment of refractory schizophrenia and severe depression; also studies have found that chlorpromazine is effective for some coronaviruses (RNA viruses), cryptococcus (fungi), Both Escherichia coli and Salmonella (bacteria) growth were inhibited to some extent. However, the 2018 annual report of the U.S. Poison Control Center shows that the death cases of sedative hypnotic / antipsychotic drug poisoning rank first. As a therapeutic drug with a strong central sedative effect and easy to obtain prescriptions, there is a large overlap in the blood concentration thresholds of poison...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72G01N30/86G16B40/10
CPCG01N30/02G01N30/72G01N30/8693G16B40/10G01N2030/027Y02A50/30
Inventor 文迪马春玲白锐谢冰
Owner HEBEI MEDICAL UNIVERSITY
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