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TLR4-based protective action mechanism for acute liver injury model caused by sepsis

A technology for acute liver injury and protection, applied in the medical field, can solve the problem of acute liver injury without effective treatment measures, etc.

Inactive Publication Date: 2021-08-13
国药葛洲坝中心医院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the existing defects and provide a protective mechanism based on TLR4 on the acute liver injury model caused by sepsis, so as to solve the current acute liver injury caused by sepsis proposed in the above background technology. There is still no effective treatment for the injury, and finding effective drugs for the treatment of acute liver injury has become an urgent problem in critical care medicine. Therefore, finding new targets is of great significance to the clinical treatment of sepsis

Method used

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  • TLR4-based protective action mechanism for acute liver injury model caused by sepsis
  • TLR4-based protective action mechanism for acute liver injury model caused by sepsis

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Experimental program
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Effect test

Embodiment 1

[0053] see figure 1 , the present invention provides a technical solution: based on the protective mechanism of TLR4 on the acute liver injury model caused by sepsis, comprising the following steps:

[0054] Step 1: Construction of mouse Sepsis model and grouping;

[0055] Step 2: miR-214 expression lentiviral vector construction;

[0056] Step 3: miR-214 intervention;

[0057] Step 4: Sample collection and testing;

[0058] Step 5: Statistical processing.

[0059] The working principle and application process of the present invention: using the mouse model of liver injury caused by sepsis, observe the changes of serological indicators, inflammatory factors and pathological tissue of mice in each group of mice after miR-214 intervention, and the changes of liver tissue. miR-214 and TLR4 signaling pathway protein expression to explore the effect of regulating miR-214 expression on mice, whether miR-214 expression can inhibit TLR4 signal transduction pathway, block inflammat...

Embodiment 2

[0061] see Figure 1-2 , the present invention provides a technical solution: based on the protective mechanism of TLR4 on the acute liver injury model caused by sepsis, comprising the following steps:

[0062] Step 1: Construction of mouse Sepsis model and grouping;

[0063] Step 2: miR-214 expression lentiviral vector construction;

[0064] Step 3: miR-214 intervention;

[0065] Step 4: Sample collection and testing;

[0066] Step 5: Statistical processing.

[0067] In the present invention, preferably, step 1 includes the following steps:

[0068] Step 1.1: Adopt SPF grade C57BL / 6 mice, female, 13 weeks old, 80 mice, body weight 20-25g, rearing temperature is 22-25°C, indoor humidity is 35%-40%, and the breeding room is regularly sterilized by ultraviolet irradiation , fed according to the standard of laboratory mice, allowed to move freely, and the feeding time was 1 week;

[0069] Step 1.2: The selected 75 mice were randomly divided into normal group, sham operation...

Embodiment 3

[0081] see Figure 1-2 , the present invention provides a technical solution: based on the protective mechanism of TLR4 on the acute liver injury model caused by sepsis, comprising the following steps:

[0082] Step 1: Construction of mouse Sepsis model and grouping;

[0083] Step 2: miR-214 expression lentiviral vector construction;

[0084] Step 3: miR-214 intervention;

[0085] Step 4: Sample collection and testing;

[0086] Step 5: Statistical processing.

[0087] In the present invention, preferably, step 1 includes the following steps:

[0088] Step 1.1: Adopt SPF grade C57BL / 6 mice, female, 13 weeks old, 80 mice, body weight 20-25g, rearing temperature is 22-25°C, indoor humidity is 35%-40%, and the breeding room is regularly sterilized by ultraviolet irradiation , fed according to the standard of laboratory mice, allowed to move freely, and the feeding time was 1 week;

[0089] Step 1.2: The selected 75 mice were randomly divided into normal group, sham operation...

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Abstract

The invention discloses a TLR4-based protective action mechanism for an acute liver injury model caused by sepsis. The TLR4-based protective action mechanism comprises the following steps: step 1, constructing a mouse Sepsis model and grouping; step 2, constructing an miR-214 expression lentiviral vector; step 3, performing miR-214 intervention; step 4, collecting and detecting a sample; and step 5, carrying out statistical processing. By discussing the influence of regulation and control over the expression of the miR-214 on mice and whether the overexpression of the miR-214 can inhibit a TLR4 signal transduction pathway or not, the inflammatory reaction is blocked, and the acute liver injury caused by sepsis is improved; and influence of regulation and control over the expression of the miR-214 on the mice is researched on the basis of the TLR4 signal transduction pathway, a reference is provided for clinical treatment of the disease, and the TLR4-based protective action mechanism has important significance on clinical treatment of sepsis.

Description

technical field [0001] The invention belongs to the field of medical technology, and specifically relates to the protective mechanism of TLR4 on acute liver injury models caused by sepsis. Background technique [0002] Sepsis is a life-threatening organ dysfunction caused by an uncontrolled inflammatory response caused by an infection, which can lead to death in severe cases. Sepsis has a high morbidity and mortality rate worldwide, and is still one of the leading causes of death in the world. About 2 million to 8 million people die of sepsis every year; during the development of sepsis, The liver is one of the most vulnerable organs, and the liver plays a very important role in sepsis: the liver is not only an important line of defense against microbial attack, but also an important target organ for damage caused by inflammatory disorders; acute liver injury can occur in sepsis During septic shock, the liver microcirculation is in a state of hypoperfusion, and liver tissue...

Claims

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Application Information

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IPC IPC(8): A01K67/027C12N15/867C12Q1/6851G01N33/68
CPCA01K67/027C12N15/86C12Q1/6851G01N33/6869G01N33/6863G01N33/6893A01K2227/105A01K2267/03A01K2207/30C12N2740/15043C12N2800/107G01N2333/525G01N2333/5412G01N2333/545G01N2333/54G01N2333/70596G01N2800/085C12Q2525/207C12Q2545/114
Inventor 邹昱琳段小勇王晟刘兴态
Owner 国药葛洲坝中心医院
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