Method for inducing haematococcus pluvialis cell type conversion and astaxanthin accumulation by using seawater
A technology of Haematococcus pluvialis and type conversion, applied in the biological field, can solve the problems of affecting the yield of astaxanthin, large errors, inadequate conversion conditions, etc., and achieve the effect of saving materials and resources
Pending Publication Date: 2021-08-13
HAINAN SANYUANXING BIOTECHNOLOGY CO LTD
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Problems solved by technology
[0003] As far as the current astaxanthin accumulation process is concerned, the final output of astaxanthin will be affected by many factors, and the control of the conversion conditions is not in place, and the large error will also affect the output of astaxanthin, such as the choice of induction conditions, the light source Selection, determination of self-conditional factors, etc.
Method used
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Experimental program
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Embodiment 1
[0042] The process of using seawater to induce the cell type conversion of Haematococcus pluvialis and to accumulate astaxanthin comprises the following steps:
[0043] Sp1: Materials and reagents are prepared using 5mg / L boron and 50-60ug / L molybdenum configuration solution, and then mixed with water at a ratio of 1:4 to form a mother liquor, and the filtered seawater is added to the culture medium and mixed with the mother liquor, and the prepared The pH in the mixed medium was adjusted to 8, the inoculum size was 10%, and the temperature was 22°C, the light intensity was 2000lx-2500lx, and the photoperiod was 12:12, and cultured for 8 days;
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The invention relates to the technical field of biology, in particular to a process for inducing haematococcus pluvialis cell type conversion and astaxanthin accumulation by using seawater, and relates to the technical field of astaxanthin accumulation, the process adopts a single-factor test and a multi-factor test, the single-factor test and the multi-factor test are combined and compared, an optimal combination of illumination intensity, transformation time and temperature is identified, and the conversion condition is more beneficial to astaxanthin accumulation, so that the accumulation content of astaxanthin is remarkably increased, which avoids the influence of blindly adjusting the parameters required for conversion on the final astaxanthin content, and avoids the use of the same materials without maximizing the benefits, resulting in a waste of resources, costs and manpower.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to a method for using seawater to induce cell type conversion of Haematococcus pluvialis and to accumulate astaxanthin. Background technique [0002] Astaxanthin, also known as astaxanthin and lobster shell pigment, is a carotenoid and the highest grade product of carotenoid synthesis. It is dark pink in color and has a chemical structure similar to β-carotene. β-carotene, lutein, canthaxanthin, lycopene, etc. are all intermediate products of carotenoid synthesis, so in nature, astaxanthin has the strongest antioxidant activity. Widely present in the biological world, especially in the feathers of shrimp, crab, fish, algae, yeast and birds, it is one of the main carotenoids in marine organisms. Astaxanthin can span the blood to the brain barrier, so astaxanthin can protect the brain, central nervous system and eyes. Astaxanthin has other effects: improve physical endurance and reduce ...
Claims
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Patent Timeline
Login to View More IPC IPC(8): C12N1/38C12N13/00C12N1/12C12P23/00C12R1/89
CPCC12N1/38C12N13/00C12N1/12C12P23/00
Inventor 赵子方周永辉郭红星周尽学
Owner HAINAN SANYUANXING BIOTECHNOLOGY CO LTD
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