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A kind of albizia flower polysaccharide, preparation method and application

The technology of Albizia Julibrissin and polysaccharide, which is applied in the field of medicine, can solve problems such as unclear polysaccharide composition, and achieve the effects of uniform components, simple and easy process, and reduced expression amount.

Active Publication Date: 2022-07-29
HUAQIAO UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The research on polysaccharides from Flos Albizia Julibrissin in the above-mentioned prior art is limited to the level of crude polysaccharides, and the composition of polysaccharides is not clear. Therefore, it is necessary to carry out in-depth research on the preparation method and structure of polysaccharides from Flos Albizia Julibrissin, in order to provide a basis for the development of alternative products of natural non-toxic and side effects pharmaceutical ingredients. new object

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  • A kind of albizia flower polysaccharide, preparation method and application
  • A kind of albizia flower polysaccharide, preparation method and application
  • A kind of albizia flower polysaccharide, preparation method and application

Examples

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Effect test

Embodiment 1

[0031] Example 1 Process optimization of acid-assisted hot water extraction for extracting polysaccharide from Albizia Julibrissin

[0032] Experimental method: Weigh 1 g of Albizia Julibrissin flower buds, add corresponding distilled water according to the experimental design in Table 1, adjust the pH value, extract at a specific extraction temperature to obtain a crude extract of Albizia Julibrissin, and use the phenol sulfuric acid method to detect the polysaccharide extraction of the obtained crude extract. Rate. According to the single-factor experiment results, it was found that the extraction time had little effect on the extraction rate of total sugar, so the extraction time was fixed at 150 min. The effect of hot water extraction method on the extraction rate of Albizia Julibrissin polysaccharide, the results are shown in Table 1.

[0033] Table 1. Box-Behnken design scheme and yield for extracting total sugar from Albizia Julibrissin by acid-assisted hot water extra...

Embodiment 2

[0036] The preparation method of embodiment 2 Albizia polysaccharide

[0037] (1) Fully sun-drying the buds of Albizia Julibrissin, weigh 100g and put it into 6500mL distilled water, use hydrochloric acid to adjust the pH value to 1.45, extract in a 90°C constant temperature water bath for 150min, centrifuge at 3500r / min for 5min, and obtain the supernatant as a crude extract;

[0038] (2) The crude extract obtained in step (1) was concentrated to 150 mL under reduced pressure with a 60°C rotary evaporator, cooled and stirred while slowly adding 600mL of absolute ethanol, placed in a 4°C refrigerator for more than 10 hours, and centrifuged at 3500 r / min for 10 minutes. Take the precipitate. After the precipitate was dissolved in 100 mL of distilled water, it was frozen at -20°C for 24 hours, thawed in a 60°C water bath, and then centrifuged to remove impurities to obtain a lysate, which was then frozen and thawed for 5 times. extract;

[0039] (3) Dissolve the primary crude...

Embodiment 3

[0045] Example 3 Structural Analysis of Albizia Julibrissin Polysaccharide

[0046] Experimental methods: 1. Homogeneity and molecular weight analysis: High performance size exclusion chromatography (HPSEC) was used to identify the component homogeneity of the sample AJP, using sugar ks-804 column, ultrapure water as the mobile phase, flow rate 1mL / min, The column temperature was 50°C, and it was detected by a refractive index detector (RIU). According to the peak time of dextran standard substance on sugar ks-804 chromatographic column, a molecular weight standard curve was made, and the relative molecular mass of AJP was calculated. Take 0.5-1 mg / mL AJP aqueous solution for full spectrum scanning (190-800 nm) to check the purity of the sample.

[0047] 2. Analysis of physical and chemical properties: The compound type of the sample was analyzed by infrared spectroscopy (FTIR) method, 2 mg of sample AJP was subjected to KBr tableting, and the Nicolet Nexus 470 infrared spect...

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Abstract

The present invention discloses a polysaccharide of Albizia Julibrissin, a preparation method and application. Acid-assisted hot water extraction of polysaccharides from Albizia Julibrissin. The crude extract was subjected to ethanol precipitation and repeated freezing and thawing to obtain a primary crude extract, and then the primary crude extract was deproteinized to obtain a secondary crude extract, which was purified by column chromatography to obtain the final product. The Albizia Julibrissin polysaccharide prepared by the above method is formed by the polymerization of homogeneous polysaccharide molecules, and is composed of arabinose, galactose and galacturonic acid in equal proportions. Compared with traditional hot water extraction, the crude extract obtained by this method has a higher sugar content; and the final obtained Albizia Julibrissin polysaccharide can significantly reduce the levels of TNF-α and COX-2 in LPS-induced RAW264.7 cells. The relative mRNA expression levels of inflammatory factors and iNOS genes (P<0.05) can be used to prepare drugs for adjuvant treatment of inflammation-related central system diseases.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a polysaccharide of acacia flowers, a preparation method and an application thereof. Background technique [0002] Albizia julibrissin is Albizia julibrissin, and its dried inflorescences are often used as medicine, mainly for sedation and hypnosis, and for the treatment of restlessness. It has been reported that the decoction of Albizia Julibrissin extract has a strong hypnotic effect, but the specific composition of the compound is not clear. [0003] At present, there are few researches on the polysaccharide of Albizia julibrissin at home and abroad, and the research on the function of the polysaccharide of Albizia julibrissin is limited to the research on the antioxidant activity of its crude polysaccharides. Yuan Jianmei et al. (Response surface methodology optimization and extraction process of Albizia Julibrissin polysaccharide [J]. China Pharmacy, 2013,24(23...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P25/28
CPCC08B37/006C08B37/0003A61P25/28
Inventor 罗巅辉
Owner HUAQIAO UNIVERSITY
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