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Separation method of magnaporthe oryzae

A technology of blast fungus and separation method, which is applied in the field of plant pathology, can solve problems such as difficult operation, and achieve the effects of improving efficiency, reducing time consumption and labor consumption

Active Publication Date: 2021-08-24
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Direct picking without auxiliary technology devices is difficult to operate, and generally requires the operator to have good micromanipulation skills

Method used

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Examples

Experimental program
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preparation example Construction

[0041] 3. Preparation of Spore Sliding Loose Silk

[0042] Take an ordinary disposable water pen refill without ink after use and burn it on the flame of an alcohol lamp. The burning part of the refill will melt. Use an alcohol lamp fire extinguisher to safely extinguish the fire on the refill, and use pointed tweezers to hold a little The melted refill is gently elongated until it forms a hair-like filament. After cooling, cut off the excess filament and keep the filament about 3cm long connected to the pen holder to form an isolated blast fungus. The spores used by spores slide loose silk, and the slide loose silk tool includes sliding silk a and screw mandrel b parts, such as figure 1 As shown, the two parts are naturally connected into one body, and the key technical function is to slide the silk a. Sliding filament a is small, soft and elastic.

[0043] When separating different specimens for use, you can wipe the sliding wire a with 75% alcohol cotton balls for surface...

Embodiment 1

[0064] Adopt a kind of separation method of rice blast fungus of the present invention, isolate the blast fungus of rice blast standard sample 1, the result of separation and cultivation is as follows Figure 6 shown.

Embodiment 2

[0066] Adopt a kind of separation method of rice blast fungus of the present invention, isolate the blast fungus of rice blast standard sample 2, the result of separation and cultivation is as follows Figure 7 shown.

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Abstract

The invention discloses a separation method of magnaporthe oryzae. According to the method, a special spore slippery filament is adopted, and a single conidium is directly separated from a rice blast standard sample and is cultured to form a rice blast bacteria strain with a single genetic composition. The implementation and operation steps of the isolated culture are as follows: 1) collecting a rice blast standard sample; (2) treating a magnaporthe oryzae standard sample; (3) preparing spore slippery filaments; (4) preparing a spore dispersing plate; (5) dispersing the conidia; (6) cutting the spore-carrying sliding scattering plate; and 7) culturing conidia and forming a monospore strain. The method has the following advantages: 1) a key appliance for separating spores is simple and easy to manufacture, and the separation operation is simple and easy; 2) single spore strains can be separated and obtained at one time; 3) the separation work consumes less time and labor; and 4) the working efficiency of separating the magnaporthe oryzae and the reliability of a separation result can be obviously improved.

Description

technical field [0001] The present invention relates to phytopathological techniques. Specifically, it is a method for isolating blast fungus. Background technique [0002] Rice blast is a major disease of rice production in my country, which is caused by the infection of Magnaportheoryzae. Carrying out the biological research of rice blast fungus is an important basis for the control of rice blast. The prerequisite for carrying out biological research on Magnaporthe grisea is to have the materials of Magnaporthe grisea. General laboratories often need to isolate and obtain Magnaporthe grisea from disease standard samples. [0003] The genetic composition of the rice blast fungus population is complex, and the biological characteristics and pathogenicity of different strains are often quite different. Normal research usually needs to obtain the material of M. oryzae with a single genetic composition, or monoclonal strain material. It is generally believed in the art tha...

Claims

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Application Information

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IPC IPC(8): C12N3/00
CPCC12N3/00C12N1/14C12N1/02C12M33/02
Inventor 张容博张君成王忠文
Owner GUANGXI UNIV
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