Thymol-containing termite control medicine and application thereof
A technology of thymol and termites, which is applied in application, animal repellent, plant growth regulator, etc., can solve the problems of the complex living environment of termites, the lack of understanding of the immune mechanism of termite defense against nematodes, and the poor effect of nematodes.
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[0032] 1. Test method
[0033] 1. Thymol's ability to survive and infect nematodes
[0034] In the suspension mixed with thymol and nematode liquid, the concentration of nematodes is 200IJ / only (26667IJ / mL), and the concentrations of thymol are respectively 0.141mg / mL, 0.166mg / mL, and 0.186mg / mL (denoted as LD10, LD20, LD30), each treatment was repeated three times, and the nematode suspension (CK) without adding thymol was used as the control. Place in a dark incubator at 25±1°C for 7 days, observe every day, randomly take 100 μL of nematode suspension for each repetition, put it in a counting plate, and check the survival of nematodes under a stereoscope. Calculation: Survival rate%=(number of living nematodes÷number of total nematodes)*100%.
[0035] Pick 10 Mellonella mellonella larvae in a 9cm petri dish with two layers of filter paper, drop 1.5mL of the above mixed nematode suspension into each dish, seal it with parafilm, and place it in a dark incubator at 25±1°C for...
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