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Preparation method of ultra-small polydopamine/polydopamine cysteine nanoparticles

A technology of ultra-small polydopamine nanometer and ultra-small polydopamine, which can be applied in nanotechnology, nanotechnology, nanomedicine and other directions, and can solve problems such as difficulty in extracting and purifying neuromelanin

Active Publication Date: 2021-08-27
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to solve the existing problems of neuromelanin extraction and purification difficulties in the prior art, and to provide a mild, simple and easy preparation process, which can improve the chelating ability of metal ions, anti-oxidation and loading capacity as a drug carrier. Preparation method of ultra-small polydopamine / polydopamine cysteine ​​nanoparticles

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  • Preparation method of ultra-small polydopamine/polydopamine cysteine nanoparticles

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Embodiment 1

[0031] The invention provides a kind of preparation method of ultra-small polydopamine nanoparticles, comprising the following steps:

[0032] Weigh 119.7 mg of dopamine hydrochloride and dissolve it in a round-bottomed flask filled with 90 mL of ultrapure water (at this time, the concentration of dopamine hydrochloride is 1.33 mg / mL), fully dissolve for 5 minutes, and obtain a uniformly mixed dopamine hydrochloride solution;

[0033] Before starting the reaction, adjust the temperature of the water bath to 50°C and keep it stable, add 760 μL of 1M sodium hydroxide solution, and stir mechanically for 5 hours;

[0034] After the reaction, the product was poured into an ultrafiltration tube with a molecular weight of 30KDa, centrifuged at a speed of 6000rpm for 15min for ultrafiltration interception, and washed repeatedly until the filtrate was completely clarified to obtain polydopamine nanoparticles.

Embodiment 2

[0036] The invention provides a preparation method of ultra-small polydopamine cysteine ​​nanoparticles, comprising the steps of:

[0037] Weigh 117mg of dopamine hydrochloride and 15.444mg of cysteine ​​in a round-bottomed flask, add 90mL of ultrapure water to fully dissolve for 10min, and obtain a uniformly mixed solution of dopamine hydrochloride and cysteine;

[0038] Set the temperature of the water bath to 60°C. After the temperature stabilizes, add 840 μL of 1M sodium hydroxide solution and mechanically stir at 700 rpm for 5 hours;

[0039] After the reaction, the product was poured into an ultrafiltration tube with a molecular weight of 30KDa, and the ultrafiltration was carried out under the centrifugation condition of 6000rpm and 15min, and the polydopamine cysteine ​​nanoparticles were obtained after repeated washing several times until the filtrate was completely clarified.

[0040] figure 1 DLS characterization of ultra-small polydopamine / polydopamine cysteine ​​...

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Abstract

The invention discloses a preparation method of ultra-small polydopamine / polydopamine cysteine nano-particles, and relates to nano-materials and research on neurodegenerative diseases. In a sodium hydroxide system, a one-pot method is used for successfully preparing polydopamine and polydopamine cysteine nano-particles with the particle size of about 10nm in a manner that dopamine and dopamine and cysteine are self-oxidized under an alkaline condition. The ultra-small nano-particles have higher specific surface area, and more functional groups are exposed on the surfaces of the nano-particles, so that the chelation capability and the oxidation resistance of metal ions of the nano-particles and the loading capability of the nano-particles serving as a drug carrier are greatly improved, and the capability of the nano-particles serving as a brain treatment drug to cross a blood brain barrier can also be improved. By utilizing the structural similarity of the polydopamine and the eumelanin and the structural similarity of the polydopamine cysteine and the brown melanin, the two nano materials provide a very convenient platform for researching the degeneration mechanism of the nigra neurons in the Parkinson's disease by realizing brain administration by crossing a blood brain barrier.

Description

technical field [0001] The invention relates to the research of nanomaterials and neurodegenerative diseases, in particular to a preparation method of ultra-small polydopamine / polydopamine cysteine ​​nanoparticles. Background technique [0002] Parkinson's disease is a common neurodegenerative disease, and its symptoms are slow autonomous behavior, anxiety, depression and cognitive decline. In recent years, the prevalence of Parkinson's disease has become more and more obvious. Epidemiology shows that 15-328 people per 100,000 people suffer from it. The most obvious pathological feature of Parkinson's disease is the degeneration and death of dopaminergic neurons in the substantia nigra. Studies have found that many interrelated mechanisms in neurons are related to neuromelanin, but the mechanism of action is still unclear. The main components of natural neuromelanin are eumelanin and pheomelanin. Due to their small content and complex extraction process, it is difficult to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G73/06A61K47/34A61P25/16B82Y5/00B82Y40/00
CPCC08G73/0672A61K47/34A61P25/16B82Y5/00B82Y40/00
Inventor 孙亚楠刘骊珠王威贾丽葛东涛石巍
Owner XIAMEN UNIV
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