A digital scanning light sheet microscopy imaging system compatible with microfluidic chips

Abstract

The invention discloses a digital scanning light sheet microscopic imaging system compatible with microfluidic chips, including: an inverted microscope detection unit; a staggered support unit, including a high-level support frame set across a two-dimensional translation stage of the inverted microscope detection unit; and A low-level support frame installed on one side of the inverted microscope; the sample scanning unit, the high-level support frame that passes through the staggered support unit and is fixed to the two-dimensional translation stage of the inverted microscope detection unit, is used to fix the microfluidic chip sample and drive the microfluidic sample Scanning movement is performed along the Z-axis direction; the digital scanning light sheet illumination unit includes an illumination objective lens and a digital scanning light sheet forming component group. The present invention uses an inverted microscope and a staggered support device as a detection light path to overcome the problem of limited space and achieve long-term, large-field, high-speed, high-resolution, and high-throughput three-dimensional imaging of freely moving living organisms.

Description

technical field [0001] The invention belongs to the field of biomedical microscopic imaging, and more specifically relates to a digital scanning light sheet microscopic imaging system compatible with microfluidic chips. Background technique [0002] The light sheet microscopic imaging system is an emerging biomedical imaging system, and its illumination light path and detection light path are usually independent and orthogonal to each other. The simplest light sheet can be produced by a cylindrical lens, which belongs to static Gaussian light sheet. The light height of this kind of light sheet cannot be adjusted; it is easy to produce stripes when encountering tissues with uneven refractive index, resulting in uneven illumination; at the same time, the laser beam is compressed into a sheet shape, and the fluorescence excitation efficiency is not high enough. [0003] Ordinary light-sheet microscopes are usually incompatible with microfluidic chips, and lack the necessary co...

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Problems solved by technology

[0004] Aiming at the above defects or improvement needs of the prior art, the present invention provides a digital scanning light sheet microscopic imaging system compatible with microfluidic chips. Space, successfully combined the clamping, control, moving elements of the microfluidic chip, and the element group formed by the digital scanning light sheet, and made the digital scanning light sheet illumination optical path and detection optical path orthogonal, thereby solving the existing technology due to component installation Crowded, unable to combine digital scanning light sheet illumination light path and technical issues of microfluidic samples

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