Method for detecting glycated serum albumin by using boric acid affinity principle

A serum albumin and glycosylation technology, applied in the field of glycosylated serum albumin detection, can solve the problems of poor stability of enzyme and antibody protein, complicated steps, time-consuming, etc., and achieve the effect of stable reagents and rapid detection

Pending Publication Date: 2021-08-27
南京无书化工有限公司 +1
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Problems solved by technology

[0003] The current method for detecting the content of glycated serum albumin is complicated, or time-consuming, or the stability of enzyme and antibody protein is poor, so it has not been widely used in clinical practice

Method used

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  • Method for detecting glycated serum albumin by using boric acid affinity principle
  • Method for detecting glycated serum albumin by using boric acid affinity principle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] refer to Figure 1-2 , a method for detecting glycosylated serum albumin using the principle of boric acid affinity, characterized in that it comprises the following steps:

[0038] S1, get a certain volume of bromocresol violet solution in a cuvette;

[0039] S2, adding the serum sample to be tested into the bromocresol violet solution;

[0040] S3, obtain the total protein concentration C by measuring the absorbance at 585nm T ;

[0041] S4, adding the phenylboronic acid adsorbent to the cuvette to react with BCP-GA;

[0042] S5, waiting for the adsorbent to be completely deposited on the bottom of the cuvette;

[0043] S6, measure the absorbance at 585nm to obtain the concentration C of non-glycated albumin ng , and through the formula (C T -C ng ) / C T *100% is calculated as a percentage of serum albumin.

[0044] Wherein, steps S1, S2 and S3 are to obtain the absorbance A0 of the bromocresol violet solution at 585 nm under alkaline conditions, that is, the ...

Embodiment 2

[0061] The difference with Example 1 is:

[0062] The repetition rate of the method

[0063] Intra-batch repeatability test: Take the same serum sample and repeat the test 20 times continuously, and calculate the CV value. Batch-to-batch repeatability test: divide the same serum sample into 20 parts, store in the refrigerator, take 1 part for testing every day, for a total of 20 days, and calculate the CV value. The intra-assay CV value and the inter-assay (day) CV value should be less than 5% according to the requirements of the National Committee for Clinical Laboratory Standardization (NCCLS) documents, and the method meets the requirements.

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Abstract

The invention belongs to the technical field of glycated protein detection, and particularly relates to a method for detecting glycated serum albumin by using a boric acid affinity principle. The method is characterized by comprising the following steps: taking a certain volume of bromcresol purple solution in a cuvette; adding a serum sample to be detected into the bromcresol purple solution; obtaining the total protein concentration CT by measuring the absorbance of 585 nm; adding a phenylboronic acid adsorbent into the cuvette to react with the BCP-GA; waiting until the adsorbent is completely deposited at the bottom of the cuvette; and measuring the absorbance of 585 nm to obtain the concentration Cng of the non-glycated serum albumin, and calculating the proportion of the glycated serum albumin through a formula (CT-Cng) / CT * 100%. According to the method, the bromcresol purple is used for testing the total albumin content under the alkaline condition, the absorbance is reduced after the albumin and the bromcresol purple are combined, the albumin content and the absorbance decline value are in a linear relation, all tests are completed through the absorbance spectrophotometry, reagents are stable, detection is rapid, simple and convenient, and the method is completely compatible with general equipment in hospitals.

Description

technical field [0001] The invention relates to the technical field of detection of glycosylated serum albumin, in particular to a method for detecting glycosylated serum albumin using the principle of boric acid affinity. Background technique [0002] Over the past half century, China's economy has developed continuously and rapidly, and people's material living standards have been continuously improved. Due to unreasonable eating habits and dietary structure of a considerable number of people, coupled with lack of sufficient exercise, the incidence of diabetes has increased rapidly, especially in recent years. At present, there are 100 million diabetic patients in China, and there is no slowing down trend. If this situation is not effectively suppressed, serious social problems will arise in a few years. [0003] The current method for detecting the content of glycated serum albumin has complicated steps, or is time-consuming, or has poor stability of enzyme and antibody ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N21/31
CPCG01N33/6839G01N21/31G01N2333/765
Inventor 韩毓旺刘启迪
Owner 南京无书化工有限公司
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