Real-time fluorescent constant-temperature nucleic acid amplification detection device

Abstract

Disclosed is a real-time fluorescent constant-temperature nucleic acid amplification detection device. The real-time fluorescent constant-temperature nucleic acid amplification detection device comprises a micro-fluidic unit, a temperature control unit, an excitation light unit, a detection unit and a mounting rod, wherein a fluorescent constant-temperature nucleic acid amplification reaction is performed on a reaction solution containing a fluorescent dye in the micro-fluidic unit; the temperature control unit provides constant temperature required by nucleic acid amplification for the micro-fluidic unit; the excitation light unit generates excitation light to excite the fluorescent dye in the reaction liquid in the micro-fluidic unit, so that the fluorescent dye generates fluorescent light; the detection unit collects information of the fluorescent light generated by the reaction liquid in the micro-fluidic unit to obtain a detection result, wherein the micro-fluidic unit, the excitation light unit and the detection unit are installed on the mounting rod together; the excitation light unit and the detection unit are located above the micro-fluidic unit; and at least the position and / or angle, relative to the micro-fluidic unit, of the detection unit on the mounting rod is adjustable. The device is simple in structure, low in cost and flexible and convenient to operate and adjust, and ensures that a detection result is reliably, stably and quickly obtained.

Description

Technical field [0001] The present invention relates to the field of nucleic acid detection, more particularly to a real-time fluorescence detection apparatus thermostat nucleic acid amplification. Background technique [0002] Micro total analysis systems (Miniaturized Total Analysis Systems) since the concept was first proposed in the 1990s, then on the basis of microelectronics, micro-mechanical, biotechnology and nanotechnology, based on microfluidic technology has developed rapidly to become the world's most cutting-edge one of science and technology. Chip laboratory (Lab on chip) is currently its core technology is based on microfluidics-based microfluidic chip, also known as. Microfluidic chip technology (a Microfluidics) is the integrated sample preparation of biological, chemical, medical analysis process, reaction, separation, detection means to the basic operation of a microscale chip, the entire process be done automatically. Microfluidic chip has advantages because l...

AI Technical Summary

Problems solved by technology

Even so, the existing constant temperature nucleic acid amplification equipment has a complex structure, complex operation, high requirements for control, inflexible and convenient use, and high cost, and there is still a lot of room for optimization

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