Method for evaluating potential applicability of insect-resistant rice variety based on specific harmful genotype of brown planthopper

Abstract

The invention discloses a relationship between polymorphic site genotypes of a brown planthopper cytochrome P450CYP4C61 gene and a nicotine acetylcholine receptor alpha-7-like gene and the damage of the brown planthopper, and provides a method for evaluating the potential applicability of an insect-resistant rice variety based on a specific harmful genotype of the brown planthopper on the basis of the relationship. Compared with a conventional evaluation method, the method has the advantage that the result is more accurate and reliable. According to the method, the found genetic variation site combination can divide any brown planthopper field population into three genotypes related to the harmful property, such that the wide representativeness and the wide applicability are provided, and the insect resistance of the target rice variety is tested by using the three genotype brown planthopper population homozygous progeny; and the effectiveness of potential rice insect-resistant varieties in field application in the future can be reflected more objectively.

Description

technical field [0001] The invention belongs to the technical field of rice insect resistance phenotype identification. More specifically, it relates to a method for assessing the potential suitability of insect-resistant rice varieties based on specific lethality genotypes of brown planthoppers. Background technique [0002] The brown planthopper is one of the main pests that harm rice. Its outbreak can lead to a large reduction in rice production and seriously threaten food security. The breeding and promotion of insect-resistant rice varieties is considered to be one of the important means to control the damage of brown planthopper and ensure food security, and it has the advantages of economy, environmental protection and sustainability. [0003] At present, in the identification process of rice insect resistance phenotype, the genetic background of the pest itself is often not required. For example, Chinese patent CN 112083128A discloses a method for phenotypic identi...

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Problems solved by technology

Chinese patent CN 111264320A discloses a method for accurate identification of rice brown planthopper resistance at the adult plant stage. The identification process requires manual inoculation, but there is no special requirement for inoculated pests

The varieties bred in this way are quickly adapted to the field population after being applied in the field, and even do not have the proper resistance phenotype to the field population at all, which cannot truly reflect the true resistance of the selected variety to the field brown planthopper population level, resulting in less than expected end-use effects

However, if the field population is simply used to test rice insect resistance, it is often difficult to obtain reliable and ideal results without knowing the genetic background of the field population's pathogenicity.

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