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A method for assessing the potential applicability of insect-resistant rice varieties based on brown planthopper-specific virulence genotypes

A genotype, brown planthopper technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as no special requirements for pests, artificial inoculation, and no resistance phenotype in field populations, reaching a wide range of representatives. Applicability and applicability, accurate and reliable results

Active Publication Date: 2022-07-12
SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent CN 111264320A discloses a method for accurate identification of rice brown planthopper resistance at the adult plant stage. The identification process requires manual inoculation, but there is no special requirement for inoculated pests
The varieties bred in this way are quickly adapted to the field population after being applied in the field, and even do not have the proper resistance phenotype to the field population at all, which cannot truly reflect the true resistance of the selected variety to the field brown planthopper population level, resulting in less than expected end-use effects
However, if the field population is simply used to test rice insect resistance, it is often difficult to obtain reliable and ideal results without knowing the genetic background of the field population's pathogenicity.

Method used

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  • A method for assessing the potential applicability of insect-resistant rice varieties based on brown planthopper-specific virulence genotypes
  • A method for assessing the potential applicability of insect-resistant rice varieties based on brown planthopper-specific virulence genotypes
  • A method for assessing the potential applicability of insect-resistant rice varieties based on brown planthopper-specific virulence genotypes

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Experimental program
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Embodiment 1

[0038]Example 1 Harmful genotype analysis of brown planthopper field populations in different regions

[0039] In order to verify the combination of genetic variation sites found in the present invention, any N. lugens field population can be divided into three genotypes related to virulence, which has extremely wide representation and applicability. The inventors randomly sampled the field population of N. lugens in Shaoguan, Guangdong, Zunyi, Guizhou, and Zhoushan, Zhejiang in 2017, and analyzed the harmful genotypes of the collected N. lugens samples through the steps of extracting N. lugens DNA, PCR amplification and sequencing. Classification.

[0040] 1. Genomic DNA extraction of brown planthopper

[0041] The present invention uses the Genomic DNAIsolation Kit of OMEGA company to extract genomic DNA, and the steps are as follows:

[0042] (1) Take the worms and grind them in liquid nitrogen thoroughly, and add 500 μL of Buffer GTC / 2-Me;

[0043] (2) Vortex vigorously...

Embodiment 2

[0071] Example 2 Method for assessing the potential applicability of insect-resistant rice varieties based on the specific virulence genotypes of the brown planthopper

[0072] A method for evaluating the potential applicability of insect-resistant rice varieties based on the specific virulence genotypes of the brown planthopper. The technical roadmap is shown in figure 1 shown.

[0073] 1. Treatment of brown planthopper field population;

[0074] The N. lugens field populations P-SG were captured in the field of Shaoguan, Guangdong, respectively, and placed on the susceptible rice variety TN1 to propagate for one generation.

[0075] After the worms grow into adults, the female adults and male adults are paired in pairs and transferred to TN1 rice in the middle stage of tillering to be reared separately. After ensuring that the female worms mate with the male worms and lay eggs for 7 days, the parental adult worms are removed for subsequent use. Genomic DNA extraction.

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Abstract

The invention discloses the relationship between the polymorphic site genotype of the brown planthopper cytochrome P450CYP4C61 gene and the nicotinic acetylcholine receptor α-7-like gene and the harm of the brown planthopper, and on this basis provides a specific harm of the brown planthopper based on the A method for assessing the potential applicability of insect-resistant rice varieties by sex genotype is more accurate and reliable than conventional assessment methods. The combination of genetic variation sites found in the present invention can divide any N. lugens field population into three genotypes related to virulence, and has extremely wide representation and applicability. Testing the insect resistance of target rice varieties can more objectively reflect the effectiveness of potential rice insect-resistant varieties when applied in the field in the future.

Description

technical field [0001] The invention belongs to the technical field of rice insect-resistant phenotype identification. More specifically, it relates to a method for assessing the potential applicability of an insect-resistant rice variety based on a specific virulence genotype of the brown planthopper. Background technique [0002] Brown planthopper is one of the main pests that harm rice, and its outbreak can lead to a large reduction in rice field production and seriously threaten food security. The selection and promotion of insect-resistant rice varieties is considered to be one of the important means to control brown planthopper damage and ensure food security, and has the advantages of economy, environmental protection and sustainability. [0003] At present, in the identification process of rice insect-resistant phenotype, the genetic background of the pest itself is often not required. For example, Chinese patent CN 112083128A discloses a method for phenotypic iden...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6895
CPCC12Q1/6888C12Q1/6895C12Q2600/156C12Q2600/13
Inventor 庞锐张文庆袁龙宇陈伟文李诗蕙
Owner SUN YAT SEN UNIV
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