Unlock instant, AI-driven research and patent intelligence for your innovation.

Immunogenic composition for paratuberculosis

A technology for vaccines and diseases, applied in the direction of bacterial antigen components, biochemical equipment and methods, medical preparations containing active ingredients, etc., can solve problems such as incomplete destructive effects and risks

Pending Publication Date: 2021-08-31
HAV VACCINES
View PDF13 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Furthermore, MAP is a hardy environmental organism, and pasteurization does not fully destroy it
Therefore, dairy products from cows, sheep and goats pose a risk to humans

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Immunogenic composition for paratuberculosis
  • Immunogenic composition for paratuberculosis
  • Immunogenic composition for paratuberculosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0316] Example 1: hAd5 HAV priming and MVA Efficacy of HAV booster vaccination in calves

[0317] St George's University of London, The Jenner Institute University of Oxford, The Roslin Institute University of Edinburgh, Animal Health and Welfare and the Agri-Food and Biosciences Institute of Northern Ireland, a BBSRC-funded trial of HAV vaccination against experimental MAP infection (2010-2014).

[0318] The amino acid sequence of the HAV vaccine with inserted leader peptide and PK tail (shown in bold) at either end is shown below. Sequences from its four MAP genes 1589c (AhpC), 1234 (Gsd), 2444c (pl2) and 1235 (mpa) as described above contain peptide sequences in between. Junctions between HAV vaccine components are marked with *.

[0319]

[0320]The underlined sequence is that of p12, the carboxy-terminal region of the P900 protein encoded by the plus strand of the IS900 element. The p12 portion within HAV has a short cytoplasmic domain followed by a highlighte...

Embodiment 2

[0329] Example 2: Stages of development of MAP antibodies

[0330] Phase 1. Profiling of mouse antibodies that bind the peptide domain in P900

[0331] IS900 (NCBI entry: AE016958.1) is a DNA insertion element of 1451 bp to 1453 bp found in three Crohn's disease isolates of MAP by the inventor and colleagues at the end of 1985 (E.Green et al Nucleic AcidsResearch1989; 17:9063-73). This DNA insertion element exists in MAP in 14 to 18 identical copies inserted at highly conserved sites throughout the MAP genome. This multicopy element has its own promoter, is abundantly expressed in humans, and causes a wide range of pathogenic phenotypes.

[0332] The positive strand of IS900 is predicted to be a protein of 406 amino acids (P900). The full-length amino acid sequence of this protein is unique to MAP, but there are P900 "analogues" covering most of the P900 molecule in closely related mycobacteria and actinomycetes.

[0333] The full-length P900 protein encoded by the plus ...

Embodiment 3

[0373] Example 3: Diagnostic techniques for detection and characterization of MAP infection in samples from humans and animals and in food use in product safety

[0374] 1. Detection and Measurement of Human Intestinal Tissue Infected with MAP

[0375] Endoscopic biopsies from 45 people with Crohn's disease and some other disorders such as irritable bowel syndrome were studied (Scanu et al. Mycobacterium avium subspecies paratuberculosis infection in cases of Irritable Bowel Syndrome and comparison with Crohn's disease and Johne's disease: common neural and immune pathogenicities. J Clin Microbiol 2007:45:3883-90). Specimens were immediately fixed in formalin, followed by standard processing and embedded in paraffin histopathology blocks. Preliminary work was performed to identify 2 μm sections as optimal. Sections were processed with standard antigen retrieval protocols. Then stain with monoclonal antibody dilutions ranging from 1 / 500 to 1 / 5000. Use direct fluorophore-l...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a vaccine comprising a polypeptide comprising an amino acid sequence of at least 9 contiguous amino acids from the N-terminal region of MAP P900, or a polynucleotide encoding the polypeptide, for use in a method of treating or preventing MAP infection or a condition or symptom associated with MAP infection in a subject.

Description

technical field [0001] The present invention relates to the treatment or prevention of Mycobacterium avium subspecies paratuberculosis (MAP) infection, and to the treatment or prevention of diseases associated with MAP infection. Background technique [0002] MAP is a very slow-growing intracellular mycobacterial pathogen that causes systemic infection and chronic intestinal inflammation (Johne's disease (JD)) in many animal species, including primates. MAP was first identified in 1894 on a sick cow in Germany. In the ensuing years, MAP infection and disease appeared to be limited to Europe and North America. Since then, the virus has spread globally due to international trade in subclinically infected livestock and the lack of reliable and sensitive diagnostic techniques capable of identifying MAP infection at its earliest stages. The MAP genome is GC-rich (69.3%) and is 96.4% homologous in sequence and genetic organization to the closely related Mycobacterium avium and o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K39/04C07K14/35
CPCC07K14/35A61K39/04C12N9/1025C12N9/1048A61P31/04A61K45/06A61K2039/5256
Inventor 约翰·赫尔蒙-泰勒
Owner HAV VACCINES
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com