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Application of hesperetin and/or derivative thereof in preparation or serving as medicine for preventing and/or treating renal cell carcinoma

A technology for renal cell carcinoma and citrus peelin, which is applied in the field of biomedicine, can solve the problem of non-universal use of tumor drugs, and achieve the effects of preventing and/or treating renal cell carcinoma, inhibiting proliferation, and improving renal cell carcinoma.

Pending Publication Date: 2021-09-07
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT) +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recent studies have shown that tangeretin, as one of the flavonoids, has anti-proliferation, anti-invasion, anti-metastasis and anti-oxidation activities, and can also inhibit the proliferation of tumor cells such as breast cancer and oral cancer, as disclosed in patent CN201510590605.5 The application of tangeretin in the preparation of drugs or health products for the prevention and treatment of oral cancer, but the pathogenic mechanisms of different cancers are completely different, and the drug for tumors is not universal, and can be applied to the preparation of drugs for the prevention and treatment of oral cancer It may not be applicable to the preparation of drugs for the prevention and / or treatment of renal cell carcinoma, and whether tangeretin has an effect on renal cell carcinoma has not been reported in relevant studies

Method used

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  • Application of hesperetin and/or derivative thereof in preparation or serving as medicine for preventing and/or treating renal cell carcinoma
  • Application of hesperetin and/or derivative thereof in preparation or serving as medicine for preventing and/or treating renal cell carcinoma
  • Application of hesperetin and/or derivative thereof in preparation or serving as medicine for preventing and/or treating renal cell carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Example 1 Analysis of Cx43 expression, correlation and its relationship with prognosis of renal cell carcinoma

[0072] Except for a few cells (such as sperm, platelets, etc.), almost all cells in mammals are connected to each other through gap junctions (Gap junction, GJ). Small molecules below 1-1.5kDa, such as calcium ions, IP3, cAMP and free amino acids, can pass between cells through GJ, so that cells can share some small molecule metabolites and regulate intercellular signal transmission. Some microRNAs (miRNAs) can also be delivered from cells to neighboring cells via GJs. Cx43 is one of the most abundant junction proteins in the GJ formed between cells, and it is related to the occurrence and development of various diseases such as ischemia-reperfusion injury, inflammation, diabetes, and tumors. However, the expression of Cx43 in renal cell carcinoma has rarely been reported. On this basis, we further explored the relationship between Cx43 and the progression o...

Embodiment 2

[0078] Example 2 Inhibition of Tangeretin on Cx43 Expression and Renal Cancer Cells

[0079] 1. Experimental method

[0080] (1) According to the method in the aforementioned "Detection of protein expression by Western blot", after collecting clinical tissue samples and different renal cell samples from patients with renal cancer, protein extraction and sample preparation were performed, and the expression of proteins such as Cx43 and EGFR were detected by western blot ; and use the method in the aforementioned "clonal formation experiment", "cell scratch method to detect cell migration ability", "Transwell chamber culture method to detect cell invasion ability" to detect the ability of cell clone formation, migration and invasion.

[0081] (2) Inoculate the cells into a 96-well plate at a certain density (about 3000-5000 cells per well), and after 24 hours for the cells to adhere to the wall, change the medium and add 11 kinds of traditional Chinese medicine monomers (1-Cyclo...

Embodiment 3

[0089] Example 3 Inhibition of Tangeretin on the Expression of Renal Cancer Cell Proliferation, Cloning, and Migration-Related Molecules

[0090] 1. Experimental method

[0091] According to the method in the aforementioned "Detection of protein expression by Western blot", the small molecule interfering RNA of Cx43 and tangeretin were used to treat and administer different renal cancer cells, and the effects of tangeretin and Cx43 expression on the proliferation, Cloning, migration closely related molecules (phosphorylated EGFR, AR, MMP-9, SOD-1, CD44, phosphorylated ERK, phosphorylated Stat3, BCL-2, P65, phosphorylated AKT and Survivin) expression.

[0092] 2. Experimental results

[0093] After inhibiting the expression of Cx43 in ACHN cells with tangeretin, the expressions of phosphorylated EGFR, AR, MMP-9 and SOD-1 were downregulated ( Figure 7 A), and inhibited the expression of CD44 in ACHN cells ( Figure 7 B); Tangeretin can inhibit the expression of phosphorylate...

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Abstract

The invention provides application of hesperetin and / or a derivative thereof in preparation or serving as a medicine for preventing and / or treating renal cell carcinoma. The application has the advantages that the hesperetin is found to be capable of effectively inhibiting renal cell carcinoma cell activity, inhibiting proliferation, cloning and migration of renal cell carcinoma cells and inhibiting expression of Cx43 in the renal cell carcinoma cells through research for the first time, and the hesperetin and the derivative thereof are indicated to have good preventing and treating effect on the renal cell carcinoma; the invention provides new application of the hesperetin and the derivative thereof and also provides a new selection scheme for targeted therapy of the renal cell carcinoma.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. More specifically, it relates to the application of tangeretin and / or its derivatives in the preparation or as a drug for preventing and / or treating renal cell carcinoma. Background technique [0002] Renal cell carcinoma (RCC, referred to as renal cell carcinoma) originates from the urinary tubular epithelial system of the renal parenchyma. It is insidious in onset and often lacks certain clinical manifestations in the early stage. It is a highly malignant tumor in the urinary system. Renal cell carcinoma accounts for about 2% to 3% of adult malignant tumors and 80% to 90% of adult renal malignant tumors. The incidence rate varies from country to country or region in the world. In general, the incidence rate in developed countries is higher than that in developing countries, and in urban areas is higher than in rural areas. There are more men than women. The ratio of male to female patients...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P35/00A61P35/04
CPCA61K31/352A61P35/00A61P35/04Y02A50/30
Inventor 赖永长吴文起梁叶萍段小鹿何朝辉梁雄发吴伟宙钟芳灵
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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