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Method for increasing content of adenosine and ergosterol in fungus mixed fermentation broth

A technology of mixed fermentation and ergosterol, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., and can solve problems that do not involve mixed fermentation of various edible fungi

Pending Publication Date: 2021-09-10
LINYI XIAOFENG MUSHROOM IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this invention is only a single edible fungus fermentation, and does not involve the mixed fermentation of multiple edible fungi

Method used

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Examples

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Effect test

Embodiment example 1

[0019] The present invention provides a technical solution: a method for increasing the content of adenosine and ergosterol in the mixed fermentation broth of fungi, which specifically includes the following steps:

[0020] (1) First prepare a variety of medicinal edible fungus PDA slant mother species, these medicinal edible fungi can be any two or Combination of more than two kinds, cultivation temperature 20~25℃, humidity 45~65%, cultivation time 7~10 days;

[0021] (2) After the mother species mature, inoculate the seed fermentation shaker flasks respectively. The volume of the seed bottle is 50mL. The seed medium is liquid PDA medium. Place 5-8 glass beads in each bottle, and the inoculation volume is controlled at 5-8 square centimeters. The culture temperature is 20~25℃, the rotation speed is 180~240rpm, and the culture time is 56~72h;

[0022] (3) According to the changing law of the growth curve of each medicinal and edible fungus, different proportions of inoculatio...

Embodiment example 2

[0026] A method for increasing the content of adenosine and ergosterol in a fungal mixed fermentation broth, further comprising the following steps:

[0027] (1) First prepare a variety of medicinal edible fungus PDA slant mother species, these medicinal edible fungi are two combinations of Ganoderma lucidum and Cordyceps, the cultivation temperature is 23 ° C, the humidity is 50%, and the cultivation time is 7 days;

[0028] (2) After the mother species mature, inoculate the seed fermentation shaker flasks respectively. The liquid volume of the seed bottles is 50mL, and the seed medium is liquid PDA medium. Place 5 glass beads in each bottle, and the inoculation volume is controlled at 5 square centimeters, and the culture temperature is 23°C. , rotation speed 180rpm, culture time 72h;

[0029] (3) According to the changing law of the growth curve of each medicinal and edible fungus, different proportions of seed liquids were mixed to inoculate fermentation shake flasks. The ...

Embodiment example 3

[0033] A method for increasing the content of adenosine and ergosterol in a fungal mixed fermentation broth, further comprising the following steps:

[0034] (1) First prepare a variety of medicinal edible fungus PDA slant mother species, these medicinal edible fungi are three combinations of Cordyceps, Golden Ear and Grifola frondosa, the cultivation temperature is 25°C, the humidity is 65%, and the cultivation time is 7 days;

[0035] (2) After the mother species mature, inoculate the seed fermentation shaker flasks respectively. The liquid volume of the seed bottles is 50mL. The seed medium is liquid PDA medium. Put 8 glass beads in each bottle. The inoculation volume is controlled at 8 square centimeters, and the culture temperature is 25°C. , rotation speed 200rpm, culture time 72h;

[0036] (3) According to the changing law of the growth curve of each medicinal and edible fungus, different proportions of seed liquids were mixed to inoculate fermentation shake flasks. The...

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Abstract

The invention discloses a method for increasing the content of adenosine and ergosterol in a fungus mixed fermentation broth, and relates to the technical field of edible fungus mixed fermentation, in particular to a method for increasing the content of adenosine and ergosterol in a fungus mixed fermentation broth, which comprises the following steps: (1) firstly, respectively preparing a plurality of medicinal and edible fungus slant mother strains at the culture temperature of 20-25 DEG C and with the humidity of 45-65% and the culture time of 7-10 days; and (2) after the mother strains are mature, respectively inoculating seed fermentation shake flasks, placing 5-8 glass beads in each flask, and controlling the inoculation amount to be 5-8 square centimeters, wherein the culture temperature is 20-25 DEG C, the rotating speed is 180-240 rpm, and the culture time is 56-72 hours. According to the invention, when mixed culture of medicinal and edible fungi is studied, it is found that the content of adenosine and sterol can be remarkably increased by combining static culture and dynamic culture; and then, the method for increasing the content of adenosine and sterol is further optimized in the invention.

Description

technical field [0001] The invention relates to the technical field of mixed fermentation of edible fungi, in particular to a method for increasing the content of adenosine and ergosterol in the mixed fermentation liquid of fungi. Background technique [0002] There is no uniform and standardized definition of medicinal edible fungi, which generally refers to microbial fungi that not only have the functions of prevention, health care and treatment of human diseases, but also can be used daily. Medicinal edible fungi have a long history of medicinal and edible use in my country. With the advancement of modern biotechnology and medical research, the efficacy of medicinal edible fungi in disease prevention, health care and treatment has been widely confirmed, and many well-known clinical medicines and healthcare products. Modern studies have shown that the main physiologically active substances contained in medicinal edible fungi are polysaccharides, terpenoids, nucleosides, st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N13/00C12P19/40C12P33/00C12R1/645
CPCC12N1/14C12N13/00C12P19/40C12P33/00
Inventor 孙效峰陈思洁徐建萍孙春晓张丽刘雪婷高士友
Owner LINYI XIAOFENG MUSHROOM IND
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