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Collagen dermal implant and preparation method thereof

A technology of collagen and implants, applied in the field of medical cosmetology, which can solve the problems of insufficient space physical support capacity, lack of natural collagen triple helix structure, etc.

Active Publication Date: 2021-09-14
尚诚怡美(成都)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Since it is not possible to simulate the in vivo process in vitro at this stage to realize the self-organization of three peptide chains to form a triple helix structure, the fermented collagen does not have the triple helix structure of natural collagen, and the spatial physical support capacity is insufficient.

Method used

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  • Collagen dermal implant and preparation method thereof
  • Collagen dermal implant and preparation method thereof

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Embodiment 1

[0028] The embodiment of the present invention provides a collagen dermal implant and a preparation method thereof, comprising the following steps:

[0029] (1) Collagen dissolution: 100 g of collagen was weighed, and the collagen raw material was commercially available, and the human-like collagen produced by eukaryotic fermentation of Jiangsu Jiangshan Juyuan was used. Add 800 ml of ultrapure water and stir magnetically for 2 hours at 4°C to obtain a homogeneous collagen gel;

[0030] (2) Mixing of reactants: at 4°C, add 2 ml of glutaraldehyde (concentration of 50%), stir magnetically for 2 hours at 4°C, and refrigerate for 4 hours;

[0031] (3) Cross-linking reaction: heating in a water bath at 45° C. for 8 hours to obtain a cross-linked collagen gel;

[0032] (4) blocking reaction: add 1500ml of ammonia water physiological saline (ammonia concentration is 2%) in the reaction system, and react for 2 hours;

[0033] (5) Purification: Purify with physiological saline, 1000m...

Embodiment 2

[0038] (1) Collagen dissolution: 100 g of collagen was weighed, and the collagen raw material was commercially available, and the human-like collagen produced by eukaryotic fermentation of Jiangsu Jiangshan Juyuan was used. Add 800 ml of ultrapure water and stir magnetically for 2 hours at 4°C to obtain a homogeneous collagen gel;

[0039] (2) Mixing of reactants: at 4°C, add 1 ml of glutaraldehyde (concentration of 50%), stir magnetically for 2 hours at 4°C, and refrigerate for 4 hours;

[0040] (3) Cross-linking reaction: heating in a water bath at 45° C. for 8 hours to obtain a cross-linked collagen gel;

[0041] (4) blocking reaction: add 1500ml of ammonia water physiological saline (ammonia concentration is 2%) in the reaction system, and react for 2 hours;

[0042] (5) Purification: Purify with physiological saline, 1000ml of physiological saline each time, 1 hour each time, purify 4 times;

[0043] (6) Granulation: the cross-linked collagen gel is sieved and granulate...

Embodiment 3

[0047] (1) Collagen dissolution: 100 g of collagen was weighed, and the collagen raw material was commercially available, and the human-like collagen produced by eukaryotic fermentation of Jiangsu Jiangshan Juyuan was used. Add 800 ml of ultrapure water and stir magnetically for 2 hours at 4°C to obtain a homogeneous collagen gel;

[0048] (2) Mixing of reactants: at 4°C, add 2 ml of glutaraldehyde (concentration of 50%), stir magnetically for 2 hours at 4°C, and refrigerate for 4 hours;

[0049] (3) Cross-linking reaction: heating in a water bath at 45° C. for 8 hours to obtain a cross-linked collagen gel;

[0050](4) blocking reaction: add 1500ml of ammonia water physiological saline (ammonia concentration is 2%) in the reaction system, and react for 2 hours;

[0051] (5) Purification: Purify with physiological saline, 1000ml of physiological saline each time, 1 hour each time, purify 4 times;

[0052] (6) Granulation: the cross-linked collagen gel is sieved and granulated...

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Abstract

The invention discloses a collagen dermal implant and a preparation method thereof. The method comprises the following steps of dissolving collagen in ultrapure water; performing refrigeration; adding a chemical cross-linking agent; performing uniform stirring at a low temperature; performing refrigeration; performing heating in a water bath to start a cross-linking reaction, so as to prepare cross-linked collagen gel ;adding normal saline-ammonia water into the cross-linked collagen gel, and performing a blocking reaction; adding normal saline into the cross-linked collagen gel after the blocking reaction for purification; granulating the purified cross-linked collagen gel by a screening method; and dispersing the granulated cross-linked collagen gel particles in normal saline to prepare a cross-linked collagen suspension; filling the cross-linked collagen suspension into a pre-filling injector; and performing moist heat sterilization to prepare the collagen dermal implant. The collagen obtained through fermentation has a physical filling function through the cross-linking reaction, and in addition, the collagen dermal implant is free of immunotoxic reaction, has good biocompatibility and can be used as a body tissue filler in medical cosmetology.

Description

technical field [0001] The invention belongs to the technical field of medical beauty, and particularly relates to a collagen dermal implant and a preparation method thereof. Background technique [0002] Collagen is composed of collagen molecules through various degrees of cross-linking. Collagen molecules are a class of proteins with a special structure. Collagen molecules are composed of three peptide chains, which are entangled into a unique triple helix structure. The primary structure of the three peptide chains is also unique, which is a repeating structure of G-X-Y three amino acid units. Typically, G is glycine, X is proline, and Y is hydroxyproline. Glycine is the smallest amino acid in the protein structure amino acid, containing two carbon atoms, and there is no branched chain on the alpha carbon atom. In the triple helix structure, the glycine of the three peptide chains is in the center of the triple helix structure and is arranged in a tight line. Since th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/50
CPCA61L27/24A61L27/50A61L2400/06
Inventor 洪涛徐震高钧
Owner 尚诚怡美(成都)生物科技有限公司
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