Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for promoting non-co-production of spores by arbuscular mycorrhizal fungi and application of method

A kind of arbuscular mycorrhizal fungus, non-common technology, applied in the field of promoting non-symbiotic sporulation of arbuscular mycorrhizal fungi, can solve the problems of long spore cycle and cumbersome steps, and achieve the effect of increasing production

Active Publication Date: 2021-09-17
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above method needs to use the root system as the host plant, and the arbuscular mycorrhizal fungus can only produce spores after establishing a symbiotic relationship with it, the steps are cumbersome, and the cycle of spore production is long

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for promoting non-co-production of spores by arbuscular mycorrhizal fungi and application of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The influence of embodiment 1 different culture media on the spore production of arbuscular mycorrhizal fungi under non-symbiotic conditions

[0023] 1. Experimental method

[0024] In this embodiment, the model strain of arbuscular mycorrhizal fungi (Rhizocystis heteromorpha DAOM197198) is selected to study the impact of the improved MSR medium on the spore production of arbuscular mycorrhizal fungi under non-symbiotic conditions, specific experimental methods and experiments The results are as follows:

[0025] (1) Weigh 0.3 mg of strigolactone, dissolve it in 1 mL of acetone, and prepare a 1 mM strigolactone solution.

[0026] (2) Weigh 11.4 mg of methyl jasmonate, dissolve it in 5 ml of absolute ethanol, and prepare a 10 mM methyl jasmonate solution.

[0027] (3) Weigh 1.33 g of potassium myristate, dissolve it in 10 ml of double-distilled water, and prepare a 0.5 M potassium myristate solution.

[0028] (4) Sterilize the strigolactone solution and the methyl jas...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for promoting non-co-production of spores by arbuscular mycorrhizal fungi and application of the method. Potassium myristate, strigolactone, methyl jasmonate and peptone are added into a culture medium of arbuscular mycorrhizal fungi. According to the invention, through creative exploration experiments, potassium myristate, strigolactone, methyl jasmonate and peptone are added into the spores of the arbuscular mycorrhizal fungi, the production amount of the spores of the arbuscular mycorrhizal fungi is remarkably increased, and the amount of the spores produced by a single mother spore of the arbuscular mycorrhizal fungi in addition treatment is 9 times that produced by a single mother spore of the arbuscular mycorrhizal fungi in non-addition treatment. The potassium myristate, strigolactone, methyl jasmonate and peptone are added, so that the spore production of the arbuscular mycorrhizal fungi under the non-symbiotic condition can be remarkably improved.

Description

Technical field: [0001] The invention belongs to the technical field of microorganism culture, and more specifically relates to a method for promoting non-symbiotic sporulation of arbuscular mycorrhizal fungi. Background technique: [0002] Arbuscular mycorrhizal fungi are biotrophic, symbiotic fungi that widely exist in soil, belonging to the subphylum Glomeromycotina, and can establish a symbiotic relationship with the roots of 80% of land plants. After establishing a symbiotic relationship between arbuscular mycorrhizal fungi and plants, it can greatly promote the absorption of mineral nutrients, such as phosphorus and nitrogen, by the roots of crops, and improve the stress resistance of crops, such as improving crops' resistance to nutrient deficiency, drought, heavy metal poisoning, pH stress, Tolerance of diseases and insect pests, etc., improves the growth potential of crops, thereby increasing the yield of crops and improving the quality of crops. Therefore, arbuscu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/14C12N3/00C12R1/645
CPCC12N1/14C12N3/00Y02A40/10
Inventor 朱红惠刘晓迪冯曾威姚青
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY