Method for improving in-vitro and in-vivo maturation quality of oocytes and application

A mature technology of oocytes, applied in the field of biomedicine, can solve the problems of oocyte quality decline and other problems, and achieve the effect of improving female fertility, function and quality

Inactive Publication Date: 2021-09-17
THE THIRD AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a medicine and its application method for improving the quality of oocyte maturation in vitro and in vivo. The problem of cell quality degradation is well improved

Method used

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  • Method for improving in-vitro and in-vivo maturation quality of oocytes and application
  • Method for improving in-vitro and in-vivo maturation quality of oocytes and application
  • Method for improving in-vitro and in-vivo maturation quality of oocytes and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Melatonin alleviates the performance of oxybenzone-induced oocyte maturation in vitro quality decline:

[0026] Step S1: preparing experimental animals and feeding and management;

[0027] The animals used in the examples are ICR female mice and male mice. The female mice are 6-10 weeks old, and the male mice are 10-14 weeks old. They are purchased from Guangdong Medical Experimental Animal Center and raised at a temperature of 20- In a temperature-controlled and light-controlled environment at 25°C with a light interval of 12 hours between light and dark, the experimental mice can eat and drink freely. After a week of adaptation, the experiment is carried out, and the operations are in compliance with the relevant regulations on the welfare of experimental animals;

[0028] Step S2: Prepare the main reagents and instruments for the experiment;

[0029] The main reagents and instruments include: pregnant horse serum gonadotropin PMSG, human chorionic gonadotropin hCG, ...

Embodiment 2

[0059] Melatonin relieves oxybenzone-induced decline in oocyte maturation quality in vivo:

[0060] Step SS1: using experimental mice as research objects, feeding and managing the experimental mice;

[0061] The animals used in the examples are ICR female mice and male mice. The female mice are 6-10 weeks old, and the male mice are 10-14 weeks old. They are purchased from Guangdong Medical Experimental Animal Center and raised at a temperature of 20- In a temperature-controlled and light-controlled environment at 25°C with a light interval of 12 hours between light and dark, the experimental mice can eat and drink freely. After a week of adaptation, the experiment is carried out, and the operations are in compliance with the relevant regulations on the welfare of experimental animals;

[0062] Step SS2: Prepare the main reagents and instruments used in the experiment. The main reagents and instruments include: pregnant horse serum gonadotropin PMSG, human chorionic gonadotropi...

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Abstract

The invention belongs to the technical field of biological medicine, and relates to a method for improving in-vitro and in-vivo maturation quality of oocytes and application of the method. Melatonin is directly added into an in-vitro maturation culture solution of the oocytes, female animals take in the melatonin, and the melatonin is proved to be capable of relieving quality reduction of the oocytes induced by oxybenzophenone. The application has the advantages that the in-vitro and in-vivo maturation rate of oocytes and the development rate of early embryos are increased, the spindle body morphology and the mitochondrial function are improved, the ROS level in the oocytes is reduced, the GSH level is increased, and the early apoptosis level is inhibited. The invention provides a method for improving the influence of oxybenzophenone on the quality of female animal oocytes, and the method has important significance for diagnosis and treatment of patients with abnormal maturation of oocytes caused by environmental factors clinically and in-vitro production of animal embryos.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a method and application for improving the maturation quality of oocytes in vitro and in vivo. Background technique [0002] Melatonin (melatonin, MT), whose chemical name is N-acetyl-5-methoxytryptamine, exists in mammals, including humans and other higher animals, and was first discovered in the pineal gland. In addition, many organs, tissues and cells (ovary, testis, skin, red blood cells, etc.) can synthesize and secrete a small amount of MT (see: Rodr í guez C, Puente-Moncada N, Reiter RJ, et al. Regulation of cancer cell glucose Metabolism is determinant for cancer cell fate after melatonin administration. J Cell Physiol. 2021; 236(1): 27-40.). Melatonin not only has the function of directly scavenging free radicals, but also regulates the expression of antioxidant enzymes, which plays an important role in maintaining the body's redox balance and improving the fecundity ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/075A61K31/4045A61P15/08
CPCC12N5/0609A61K31/4045A61P15/08C12N2501/30
Inventor 范勇金龙
Owner THE THIRD AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIVERSITY
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