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Fluorescent staining method for plasmodium detection

A fluorescent staining, Plasmodium infection technology, applied in fluorescence/phosphorescence, measurement device, resistance to vector-borne diseases, etc. The effect of easy identification, improved detection rate and simple operation process

Active Publication Date: 2021-09-17
广州创瑞健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to its high requirements on experimental techniques and conditions, its application in the field is limited.
[0015] The application of the Dipstick method also has certain limitations. It is difficult to detect Plasmodium falciparum that is still in the incubation period or only contains mature gametocytes in the blood.

Method used

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  • Fluorescent staining method for plasmodium detection
  • Fluorescent staining method for plasmodium detection
  • Fluorescent staining method for plasmodium detection

Examples

Experimental program
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Effect test

Embodiment Construction

[0050] Refer to the attached figure 1 , take out the Plasmodium Fluorescent Staining Solution, and shake the reagent bottle gently so that the liquid in the bottle reaches the bottom.

[0051] Refer to the attached figure 2 , take 20 μL of the blood sample to be tested on a clean glass slide; open the reagent bottle and take 10 μL on the glass slide of the object to be tested; cover with a clean cover glass for fluorescence microscope observation.

[0052] Refer to the attached image 3 and 4 , the red blood cell image with fluorescent signal, indicating that the malaria parasite is positive; the cell image without fluorescent signal, indicating that it is negative.

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Abstract

The invention relates to a fluorescent staining method for plasmodium detection and a reagent thereof. The method is characterized by comprising the following steps: obtaining a small molecule compound capable of being specifically combined with a plasmodium nuclear structure through a molecular fishing experiment, and modifying and marking the small molecule compound to form a specific fluorescein marked small molecule; by using the reagent provided by the invention, fluorescence staining detection on plasmodium in blood is realized; and after the reagent is mixed with a sample, the specific fluorescein labeled small molecules are combined with the plasmodium, so that the red blood cells containing the plasmodium are in bright color or specific color under a fluorescence microscope, and other blood cells are in black or background color.

Description

technical field [0001] The invention relates to a rapid detection method using fluorescein as a marker and a fluorescent dyeing reagent capable of detecting Plasmodium. protozoa, Plasmodium malariae, Plasmodium ovale, Plasmodium brasiliensis, and can be used in related fields of biology and medical testing. Background technique [0002] Fluorescence staining is a method of using the optical properties of fluorescein to emit a wavelength of a specific color under the excitation of a special wavelength of excitation light, and use a microscope to observe the substance to be tested. Fluorescent staining methods include non-specific fluorescent staining reagents and specific fluorescent staining reagents. [0003] Non-specific fluorescent dyeing reagents generally use non-specific fluorescent fuels, including auramine O fluorescent dyes, etc. These reagents can dye a certain biological macromolecules, but cannot stain cells, viruses, and other biological macromolecules. bacter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6439Y02A50/30
Inventor 王海郑南才
Owner 广州创瑞健康科技有限公司
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