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Application of vanillin derivatives in the preparation of drugs for the treatment of colorectal cancer complicated with Fusobacterium nucleatum infection

A technology of Fusobacterium nucleatum and derivatives, applied in the field of medicine, can solve problems such as reducing the curative effect, and achieve the effects of improving curative effect, solving drug resistance problems, and significant bacteriostatic activity.

Active Publication Date: 2022-04-08
HANGZHOU WEDO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the literature (Yu T C, Guo F, u Y, et al. Fusobacterium nucleatum Promotes Chemoresistance to Colorectal Cancer by Modulating Autophagy. Cell, 2017, 170(3):548.) discloses that after Fusobacterium nucleatum infects colorectal cancer cells, Lead to drug resistance of cisplatin, irinotecan, 5-FU and other chemotherapeutic drugs, reducing the curative effect

Method used

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  • Application of vanillin derivatives in the preparation of drugs for the treatment of colorectal cancer complicated with Fusobacterium nucleatum infection
  • Application of vanillin derivatives in the preparation of drugs for the treatment of colorectal cancer complicated with Fusobacterium nucleatum infection
  • Application of vanillin derivatives in the preparation of drugs for the treatment of colorectal cancer complicated with Fusobacterium nucleatum infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Determination of Vanillin Derivatives Anti-Fusobacterium Nucleatum Activity

[0039] In order to avoid the contamination of liquid strains, before each experiment, carry out Gram-staining on the culture liquid, and then observe it with an oil immersion lens. When it is confirmed that the Fusobacterium nucleatum is in a normal shape, pink fusiform, and free from other bacterial contamination, it can be used for Further experiments.

[0040] For the rapid counting of the bacterial suspension in subsequent experiments, the OD was first constructed 600 Standard curve between absorbance value and dilution factor, and OD 600 A standard curve between the absorbance value and the number of colonies, such as figure 1 shown, where A is OD 600 The absorbance value and the dilution factor conform to the equation y=0.0286+0.9497x, and R 2 >0.999, the linearity is qualified, and the absorbance is between 0.1-1.0, which ensures the accuracy of the absorbance value. In o...

Embodiment 2

[0083] Example 2 Determination of anti-colorectal cancer activity of vanillin derivatives

[0084] 1. Bioinformatics analysis

[0085] In this study, the GSE122182 data set was selected, and Affymetrix miRNA microarray technology was used to study the expression data of miRNA profiles in colorectal cancer infected with Fusobacterium nucleatum and surrounding normal tissues. The limma package was used for probe screening and quantile normalization preprocessing, and ggplot2 was used to construct the volcano plot. The expression differences and survival curves of the corresponding genes in healthy people and cancer patients were analyzed using the online website GEPIA (http: / / gepia.cancer-pku.cn / ).

[0086] Firstly, the GSE122182 data set was selected for analysis. This data set came from the RNA sequencing of colorectal cancer tissues infected by Fusobacterium nucleatum and its adjacent normal tissues, and the genes whose expression was changed after Fusobacterium nucleatum in...

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Abstract

The invention belongs to the field of medicine, and in particular relates to the application of a vanillin derivative in the preparation of a drug for treating colorectal cancer complicated with Fusobacterium nucleatum infection. The present invention unexpectedly finds that the vanillin derivatives IPM711 and IPM712 can destroy the cell wall structure of Fusobacterium nucleatum, and have significant antibacterial activity against Fusobacterium nucleatum; the vanillin derivatives IPM711 and IPM712 can be combined In the case of Bacillus infection, it can more effectively inhibit the metastasis and invasion of colorectal cancer, and inhibit the expression of related genes in HCT116 cancer cells infected by Fusobacterium nucleatum; Rectal cancer has the problem of drug resistance, and the IPM711 and IPM712 described in the present invention can significantly improve the curative effect of colorectal cancer combined with Fusobacterium nucleatum infection, solve the problem of drug resistance caused by Fusobacterium nucleatum infection, and can be used for the treatment of colorectal cancer. Rectal cancer complicated with Fusobacterium nucleatum infection.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the application of a vanillin derivative in the preparation of a drug for treating colorectal cancer complicated with Fusobacterium nucleatum infection. Background technique [0002] Colorectal cancer (CRC) is the third most common cancer and the second leading cause of cancer-related death worldwide. The purpose of chemotherapy for patients with advanced colorectal cancer is to reduce tumor volume, reduce tumor growth, and inhibit tumor metastasis. Commonly used drugs include 5-fluorouracil (5-FU), capecitabine, oxaliplatin, cisplatin, irinotecan, etc., among which 5-fluorouracil (5-FU) and capecitabine are used to inhibit DNA replication The activity of thymidylate synthase in the process; Oxaliplatin inhibits the growth of tumor cells and causes cell G2 phase arrest by covalently binding DNA and forming platinum-DNA adducts. Combinations of these chemotherapeutic agents a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4745A61P35/00A61P31/04A61P35/04
CPCA61K31/4745A61P35/00A61P31/04A61P35/04
Inventor 陈朋周忠坤胡树坚马云浩张仁涛杜康嘉张豪孙梦泽江欣荣涂洪源
Owner HANGZHOU WEDO BIOTECHNOLOGY CO LTD