Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of three-block polypeptide, a collagen biomimetic material formed by the self-assembly of three-block polypeptide

A triblock and polypeptide fragment technology, applied in the field of collagen biomimetic materials, can solve the problems of difficulty in introducing collagen functional sequences and cannot be used to prepare biological materials, etc., and achieves good application potential, wide applicability, and mild conditions. Effect

Active Publication Date: 2022-05-17
LANZHOU UNIVERSITY
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The latest research has found that collagen polypeptides can be spontaneously assembled to form fibrous structures through carefully designed electrostatic interactions between amino acids. However, this system has very strict requirements on the composition and sequence of amino acids, and it is difficult to introduce functional sequences of collagen. Therefore, it cannot be used to prepare biomaterials with collagen function

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of three-block polypeptide, a collagen biomimetic material formed by the self-assembly of three-block polypeptide
  • A kind of three-block polypeptide, a collagen biomimetic material formed by the self-assembly of three-block polypeptide
  • A kind of three-block polypeptide, a collagen biomimetic material formed by the self-assembly of three-block polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Preparation of Example 1 Collagen Biomimetic Material CTC'-393

[0080] 1. Preparation method

[0081] Under the condition of aqueous solution, prepare 8 mg / mL triblock polypeptide K at pH 7.0 3 (GPO) 9 D. 3 solution, the triblock polypeptide K 3 (GPO) 9 D. 3 The structural representation of figure 1 Shown in middle d-f (CTC'-393), triblock polypeptide K 3 (GPO) 9 D. 3 A triple helix structure.

[0082] triblock polypeptide K 3 (GPO) 9 D. 3 solution, heated in a water bath at 40°C for 50 minutes; stop heating, wait for the triblock polypeptide K 3 (GPO) 9 D. 3 The solution was naturally cooled to room temperature, and transferred to 25°C for constant temperature incubation for 36hrs. Triblock Peptide K 3 (GPO) 9 D. 3 The solution becomes cloudy ( image 3 CTC'-393 in the upper middle). Simultaneous triblock polypeptide K 3 (GPO) 9 D. 3 The absorption value of the ultraviolet 313nm corresponding to the assembly is 1.811 ( figure 2 shown in a), t...

Embodiment 2

[0087] Preparation of Example 2 Collagen Biomimetic Material CTC'-494

[0088] 1. Preparation method

[0089] Under the condition of aqueous solution, prepare 5 mg / mL triblock polypeptide K at pH 7.0 4 (GPO)9 D. 4 solution. Triblock Peptide K 4 (GPO) 9 D. 4 The structural characterization results of figure 1 Shown in middle d-f (CTC'-494), the triblock polypeptide K 4 (GPO) 9 D. 4 Has a triple helix structure.

[0090] triblock polypeptide K 4 (GPO) 9 D. 4 solution, heated in a water bath at 37°C for 30 minutes; stop heating, wait for the triblock polypeptide K 4 (GPO) 9 D. 4 The solution was naturally cooled to room temperature, and transferred to 25°C for constant temperature incubation for 48hrs. Triblock Peptide K 4 (GPO) 9 D. 4 The solution becomes cloudy ( image 3 shown in CTC'-494). Triblock Peptide K 4 (GPO) 9 D. 4 The absorption value of the ultraviolet 313nm corresponding to the assembly is 2.153 ( figure 2 shown in a), the dynamic light sc...

Embodiment 3

[0095] Preparation of Example 3 Collagen Biomimetic Material CTC'-595

[0096] 1. Preparation method

[0097] Under the condition of aqueous solution, prepare 10 mg / mL triblock polypeptide K at pH 7.0 5 (GPO) 9 D. 5 solution. Triblock Peptide K 5 (GPO) 9 D. 5 The structural characterization results of figure 1 As shown in g-i (CTC'-595), the three-block polypeptide K 5 (GPO) 9 D. 5 A triple helix structure.

[0098] Triblock Peptide K 5 (GPO) 9 D. 5 solution, heated in a water bath at 30°C for 40 minutes; stop heating, wait for the triblock polypeptide K 5 (GPO) 9 D. 5 The solution was naturally cooled to room temperature, and transferred to 25°C for constant temperature cultivation for 24hrs. The triblock polypeptide K 5 (GPO) 9 D. 5 The solution becomes cloudy ( image 3 shown in CTC'-595). Triblock Peptide K 5 (GPO) 9 D. 5 The absorption value of the ultraviolet 313nm corresponding to the assembly is 2.162 ( figure 2 shown in a), the dynamic light ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of collagen biomimetic materials, in particular to a triblock polypeptide and a collagen biomimetic material formed by self-assembly of the triblock polypeptide. The three-block polypeptide is a polypeptide sequence capable of forming a triple helical structure, including block T, block C and block C', and the block C and block C' are located at the N-terminal and C-terminal of the block T respectively. end; the charge of the block C and the block C' are opposite; the middle block T is suitable for any amino acid sequence capable of forming a triple helix structure, and has wide applicability. The collagen biomimetic material formed by self-assembly of the three-block polypeptide of the present invention can successfully simulate the structure and function of natural collagen, and has a simple preparation method and mild conditions, and has broad application prospects in the fields of tissue engineering, medical equipment and the like.

Description

technical field [0001] The invention belongs to the technical field of collagen biomimetic materials, in particular to a triblock polypeptide and a collagen biomimetic material formed by self-assembly of the triblock polypeptide. Background technique [0002] Collagen, as a key structural protein of the human body, has been the main target of biomimetic design in the field of tissue engineering for decades. Collagen is composed of three α-peptide chains with (Gly-Xaa-Yaa)n repeating amino acid sequence, which are tightly packed to form a unique triple helix structure. The rope-like collagen triple helix continues to self-assemble to form a periodic fiber structure, and these collagen fibers are involved in the regulation of cell adhesion, migration, differentiation and other cellular processes as well as the interaction of extracellular matrix biomolecules. Collagen fibers form a molecular scaffold that supports and protects the vital tissues and organs of the body. Collag...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00A61L27/24A61L27/50A61L27/60
CPCC07K14/78A61L27/60A61L27/24A61L27/50C07K2319/00
Inventor 肖建喜么林妍
Owner LANZHOU UNIVERSITY