Polynucleotide pair for detecting allergen component, probe combination and application of polynucleotide pair and probe combination

A polynucleotide and allergen technology, applied in the field of polynucleotide pairs and probe combinations for detecting allergen components, can solve problems such as difficulty in detection and analysis, and achieve the effects of accurate detection results, high sensitivity and low detection limit

Active Publication Date: 2021-09-24
HUBEI PROVINCIAL INST FOR FOOD SUPERVISION & TEST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As a result, multiple allergens may exist in the same food at the same time, and some allergens are contained in very small amounts, which makes detection and analysis difficult

Method used

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  • Polynucleotide pair for detecting allergen component, probe combination and application of polynucleotide pair and probe combination
  • Polynucleotide pair for detecting allergen component, probe combination and application of polynucleotide pair and probe combination
  • Polynucleotide pair for detecting allergen component, probe combination and application of polynucleotide pair and probe combination

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Embodiment 1

[0026] 1. Probe Design

[0027] The present invention designs probe sequences for detecting 19 kinds of allergen components, specifically as follows:

[0028] In the present invention, a high-copy ITS (internal transcribed sequence) sequence is selected from plant-derived species, and mitochondrial gene or 16SrDNA encoding cytochrome C oxidase is selected from animal-derived species as probes to design target sequences. Search about 10 target gene sequences for each allergen species from NCBI, and Sequencer / Geneious software performs sequence comparison, removes sequences with poor consistency, and derives the sequence after comparison; then compares the sequences of different species to find out the differences sequence, and design probes based on the target sequence. When designing the sequence, control the probe length (75-200bp), GC content (30-70%), Tm (68-80°C) value, etc. of each species, and the total length difference of probes of different species is at least 1 bp; ...

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Abstract

The invention relates to the technical field of food detection, in particular to a polynucleotide pair for detecting allergen components, a probe combination and application of the polynucleotide pair and the probe combination. The invention designs the probe combination for detecting 19 allergen components which specifically include pistacia vera L., Bertholletia excelsa, Apium graveolens L., gluten, Macadamia ternifolia F. Muell., eggs, Sesamum indicum, crabs, milk, hazelnuts, shrimps, Glycine max (Linn.) Merr., fishes, Arachis hypogaea Linn., sunflower seeds, Juglans regia L., Anacardium occidentalie Linn, almonds and mustard. By use of the probe combination, 19 allergen components in food can be accurately detected through one-time reaction by virtue of a multiplex ligation-dependent probe amplification technology, and the probe combination has important significance in the field of allergen component detection.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a polynucleotide pair for detecting allergen components, a probe combination and an application thereof. Background technique [0002] Food allergy is considered one of the five most important public health problems. With the development of clinical medical diagnostic technology, the number of life-threatening syndromes caused by food allergens is continuously increasing, and the problem of food allergy has become a prominent problem in the field of food safety. Allergens ingested by allergy sufferers can trigger a variety of immune reactions, including urticaria, itching, edema, bronchospasm, rhinitis, vomiting and diarrhea, and even fatal symptoms such as dyspnea and anaphylactic shock. Under normal circumstances, when the exposure of allergens is 1-100ppm, an allergic reaction will be triggered, but sometimes a small amount of intake can also cause sensitization. At p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6876C12Q1/6816C12N15/11
CPCC12Q1/6876C12Q1/6816C12Q2600/16C12Q2531/113C12Q2565/125
Inventor 王鸣秋张莉范志勇刘艳李诗瑶何名扬朱必婷张涛
Owner HUBEI PROVINCIAL INST FOR FOOD SUPERVISION & TEST
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