Living single cell rotation accurate active light control method and device based on double-core optical fibre

Abstract

The invention provides a living single cell rotation accurate active light control method and device based on a double-core optical fibre. The device is characterized by consisting of two double-core optical fibre light control systems and a microscopic imaging system. Two double-core optical fibres with output ends are processed into specific-angle frustums; the output end faces are opposite; and the central axes of fibre cores are perpendicular to each other and are fixedly installed, wherein the laser beam output by one double-core optical fibre forms a Bessel light field near the output end, so that stable capture of cells is realized; the other double-core optical fibre outputs laser pulses with a certain time interval; and the laser pulses act on the two ends of the captured cell respectively. The intensity and the time interval of the two pulses are adjusted; and accurate active light control over the rotation angle of the captured cells is achieved. The device can realize stable capture of specific living single cells and accurate active light control of rotation angles, has the characteristics of low cost, high flexibility, high integration level and the like, and has wide application prospects in the research fields of medicine, biology, life science and the like.

Description

(1) Technical field [0001] The present invention provides a dual-core optical fiber-based precise active light control method and device for the rotation of living single cells, which can be used to achieve stable capture of specific living single cells and precise active light control of the rotation angle around a specific rotation axis. It belongs to the research field of photodynamic manipulation. (2) Background technology [0002] Since the beginning of the new century, with the continuous advancement of scientific means, human exploration of nature has also continued to deepen, and people's understanding of biology has gradually deepened, from the study of nature to individuals, from individuals to cells, from cells to cells. Molecules, people's research is getting deeper and deeper. Among them, cells are the basic unit of life structure and function, and in-depth research on them is the key to revealing the mysteries of life phenomena, conquering diseases and transfo...

AI Technical Summary

Problems solved by technology

[0005] The traditional optical tweezers technology not only needs to use a large numerical aperture microscopic objective lens and complex optical path system, but also is limited by many factors in the use process, such as working distance and substrate compatibility. The system is bulky, expensive and flexible. poor sex

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