Oat bran fermentation product, skin external preparation containing oat bran fermentation product, and preparation method and application of oat bran fermentation product
An external preparation for skin, the technology of oat bran, which is applied in the field of fermentation, can solve the problems of high cytotoxicity, difficult methods of active ingredients, low utilization rate of oat bran, etc., and achieves improved utilization rate, good safety, and good water retention. effect of efficacy
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[0070] The preparation method of Saccharomyces cerevisiae 1 bacterium liquid in following embodiment 1 comprises the following steps as:
[0071] (1) Activation of strains: Saccharomyces cerevisiae 1 was inoculated in YPD solid medium for streak activation, cultured in an incubator at 28°C for 48 hours, and a single colony was obtained;
[0072] (2) Expansion of bacterial strains: inoculate the single colony obtained in step (1) into 100 mL of YPD liquid medium, and cultivate it in a shaking table at 28 ° C and 180 rpm for 48 h to obtain Saccharomyces cerevisiae 1 bacterial liquid with a concentration of 10 7 CFU / mL.
[0073] The preparation method of Saccharomyces cerevisiae 2 bacterium liquid and Saccharomyces cerevisiae 3 bacterium liquid in the following examples is compared with Saccharomyces cerevisiae 1 bacterium liquid, the only difference is that the strains inoculated in step (1) are respectively replaced by Saccharomyces cerevisiae 2 or Saccharomyces cerevisiae 3, ...
Embodiment 1
[0075] The preparation method of the fermentation substrate comprises the following steps: add 6 g of oat bran and 1.5 g of glucose into 300 g of water, and sterilize for 30 minutes at a temperature of 110° C. and a pressure of 0.1 MPa to obtain an oat bran fermentation substrate;
[0076] The concentration obtained by the above method is 10 7 CFU / mL of Saccharomyces cerevisiae 1 bacterium liquid and oat bran fermentation substrate were used as raw materials to prepare oat bran fermented product, which specifically included the following steps: inoculating 3g of Saccharomyces cerevisiae 1 bacterium liquid into 300g oat bran fermentation substrate , fermented and cultivated for 45 hours at a temperature of 28°C with stirring, and the stirring speed was 170rpm; the prepared material was transferred to a centrifuge, centrifuged at a speed of 4500rpm for 20min, and the supernatant was collected. Sterilize at a temperature of 100°C and a pressure of 0.1MPa for 30 minutes; after ste...
Embodiment 2
[0078] The preparation method of the fermentation substrate comprises the following steps: add 6 g of oat bran and 1.5 g of glucose into 300 g of water, and sterilize for 30 minutes at a temperature of 110° C. and a pressure of 0.1 MPa to obtain an oat bran fermentation substrate;
[0079] The concentration obtained by the above method is 10 7 CFU / mL of Saccharomyces cerevisiae 2 bacteria liquid and oat bran fermentation substrate are used as raw materials to prepare oat bran fermentation product, which specifically includes the following steps: inoculate 3g of Saccharomyces cerevisiae 2 bacteria liquid into 300g of oat bran fermentation substrate , fermented and cultivated for 45 hours at a temperature of 28°C with stirring, and the stirring speed was 170rpm; the prepared material was transferred to a centrifuge, centrifuged at a speed of 4500rpm for 2min, and the supernatant was collected. Sterilize at a temperature of 100°C and a pressure of 0.1MPa for 30 minutes; after ste...
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