Immunomagnetic bead and preparation method and application thereof

Abstract

The invention relates to an immunomagnetic bead and a preparation method and application thereof. According to the immunomagnetic bead (IMNs), an EpCAM aptamer and a PTK7 aptamer are selected to jointly serve as markers for recognizing CTCs, the CTCs are enriched and captured through an antigen aptamer affinity reaction, compared with a single aptamer serving as the marker for recognizing the CTCs, the CTCs which do not express or are low in EpCAM can be effectively obtained, the enrichment efficiency of the CTCs is very high and reaches more than 90%. The immunomagnetic bead (IMNs) prepared by the invention has good biocompatibility with CTCs to be captured, can actively capture target cells, and can be removed by degrading DNA aptamer chains, so that the target cells are easy to release, the preparation method is simple, the reaction conditions are mild, the cost is low, the application range is wide, and circulating gastric cancer cells, breast cancer cells, lung cancer cells or liver cancer cells can be enriched, separated and detected.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an immunomagnetic bead, a preparation method and an application of the immunomagnetic bead. Background technique [0002] Circulating tumor cells (CTCs), which are rare cancer cells released from tumors into the blood, are considered as markers of tumor invasion, contain all real-time molecular information of tumorigenesis and metastasis, and play a key role in tumor metastasis. At the same time, as a liquid biopsy technique, CTCs detection can reduce the chance of invasive tissue sampling, and has great potential in basic research and clinical application of tumor treatment. Therefore, quantitative detection of CTCs in patients' blood will provide timely and valuable information for early diagnosis, clinical treatment, recurrence detection and prognosis of malignant tumors. [0003] Due to the extremely rare number of circulating tumor cells in peripheral blood,...

AI Technical Summary

Problems solved by technology

However, the main defect of this system is that it can only identify and capture CTCs expressing EpCAM alone, and cannot effectively obtain CTCs that do not express or have low EpCAM

In addition, in this system, the captured CTCs will lose their original viability, and the antibodies are expensive and cumbersome to operate

[0004] Since the current CTC capture efficiency cannot reach 100%, and common CTC capture methods have certain defects in sensitivity, specificity, time and cost, etc., the preparation can not only effectively enhance the recognition and binding with CTCs, but also can operate Simple, low-cost immunomagnetic beads are a key step in the detection and characterization of CTCs

Images