Method for identifying influence mechanism of slope cropland furrow layout on microorganisms

Abstract

The invention provides a method for identifying the influence mechanism of slope cropland furrow layout on microorganisms, and belongs to the technical field of soil microbiology, and the method comprises the following steps: determining slope cropland furrow layout parameters; laying an experimental cell and a contrast cell; performing high-throughput sequencing on soil microorganisms to obtain a soil microorganism community structure diversity related index and functional gene abundance data in a soil carbon-nitrogen cycle process; determining a primary and secondary sequence of slope cropland furrow layout parameters influencing the soil microbial community structure diversity related indexes and the functional gene abundance data in the soil carbon and nitrogen cycle process; constructing a linear regression equation; and performing significance test on the linear regression equation to complete identification of the influence mechanism of the slope cropland furrow layout on the microorganisms. According to the method, four representative furrow layout parameters are selected, the diversity of soil microbial communities under different furrow layouts is analyzed through a high-throughput sequencing technology, the influence mechanism of the slope farmland furrow layout on soil microorganisms is identified, and reference is provided for formulating a proper slope farmland furrow layout mode.

Description

technical field [0001] The invention belongs to the technical field of soil microbiology, and in particular relates to a method for identifying the influence mechanism of ridge and furrow layout on slope cultivated land on microorganisms. Background technique [0002] As a common agricultural soil resource, sloping farmland seriously restricts the agricultural production of dry land due to its unique landform type. As a common conservation tillage measure, ridge and furrow farming can effectively solve the problem caused by sloping farmland under certain conditions. Running water and running fat and other issues. Microorganisms in the soil participate in material circulation and energy flow between soil ecosystems, and promote the growth and development of crop roots. At present, the research on the effect of ridge and furrow tillage on slope farmland on soil microorganisms mainly focuses on the effect of furrow tillage combined with film mulching and straw mulching on micro...

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Problems solved by technology

[0003] In view of the above-mentioned deficiencies in the prior art, the present invention provides a method for identifying the mechanism of influence of ridge and furrow layout on microorganisms in slope farmland, which solves the problem that it is impossible to simply understand whether the pattern of ridge and furrow cultivation has an impact on soil microorganisms

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