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Application of passionflower extract in preparation of medicine for preventing and treating breast cancer

A breast cancer, extract technology, applied in the application field of passion flower extract in the preparation of breast cancer drugs, can solve the problems of large adverse reactions, low water solubility, etc., to achieve good anti-breast cancer activity, inhibit proliferation, The effect of inhibiting migration

Active Publication Date: 2021-11-05
HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Discovering active drugs for treating tumors from plants has always been the research direction of global medical researchers. Paclitaxel from plants is widely used in the treatment of breast and ovarian cancer, but it has disadvantages such as low water solubility and large adverse reactions.

Method used

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  • Application of passionflower extract in preparation of medicine for preventing and treating breast cancer
  • Application of passionflower extract in preparation of medicine for preventing and treating breast cancer
  • Application of passionflower extract in preparation of medicine for preventing and treating breast cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The active ingredient extraction of embodiment 1 Passiflora vine leaves

[0033] Experimental materials: The vine leaves of P. edulis were collected from Chengmai, Haikou City, Hainan Province.

[0034] The collected vine leaves were dried in an oven at 40°C, crushed, passed through a 40-mesh sieve, soaked in 80% ethanol at a volume ratio of 1:5, and extracted by ultrasonic waves for 3 times, each time for 30 minutes. The extract is concentrated into an extract extract by rotary evaporation under reduced pressure, and the extract extract is freeze-dried to obtain a dry extract powder; the dry extract powder is dissolved in water to form a dry extract powder solution, and then the extractant petroleum ether is added for extraction. The solution was evaporated under reduced pressure to obtain the passionflower vine leaf extract.

[0035] The volume of the extractant is twice that of the extract dry powder solution. The volume ratio of described ethanol and Passiflora vi...

Embodiment 2

[0036] Example 2 The CCK-8 method was used to measure the activity of inhibiting the proliferation of breast cancer cells

[0037] The Passiflora vine leaf extract of embodiment 1 is mixed with the Passiflora vine leaf extract solution of different concentrations (31, 62.5, 125, 250, 500 μ g / mL) with DMSO solvent to carry out the cell activity test, and make the culture medium The DMSO content in it is less than 0.1%.

[0038] 96-well plate, inoculate about 4000 MDA-MB-231 cells per well, add the DMEM culture solution (containing 0.25% HEPES) of the above-mentioned different concentrations of Passiflora vine leaf extract after the cells adhere to the wall, and add 0.1% DMSO Culture medium was used as negative control. After 72 hours of treatment, add CCK-8 and put CO 2 After 1 hour in the incubator, read the absorbance at 405 nm with a microplate reader, and the cell viability=(A blank group-A treatment group) / A blank group×100%.

[0039] The experimental results show that ...

Embodiment 3

[0041] Example 3 Determination of inhibition of breast cancer cell migration activity by scratch method

[0042] 24-well plate with 2×10 per well 5Inoculate MDA-MB-231 cells in the logarithmic growth phase with a cell amount of 24 hours, scratch with the tip of a pipette after the cells adhere to the wall, wash off the floating cells with PBS, and dissolve the Passiflora vine leaf extract formulation described in Example 1 with DMSO solvent. Passiflora vine leaf extract extract (25, 50, 100, 200, 400 μg / mL) into different concentrations, stain the cells with crystal violet after 24 hours, take pictures under the microscope, count the changes in the scratch area before and after the treatment, and calculate the inhibition mobility.

[0043] The experimental results show that: different concentrations of Passiflora vine leaf extract all have the activity of inhibiting MDA-MB-231 cell migration, wherein 400 μ g / mL treatment compares the average inhibitory rate of 96.14% ( imag...

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Abstract

The invention discloses an application of a passionflower vine leaf extract in preparation of a medicine for preventing and treating breast cancer. The active ingredients in the passionflower vine leaf extract comprise isoorientin, vitexin and isovitexin flavone. The invention also discloses an extraction method of the passionflower vine leaf extract. The passionflower vine leaf extract can inhibit proliferation of breast cancer cells, can inhibit migration of the breast cancer cells, and can inhibit the cycle of the breast cancer cells; and the passionflower vine leaf extract can be used for preparing the medicine for preventing and treating breast cancer and other anti-cancer medicines.

Description

technical field [0001] The application relates to the field of application of plant extracts, in particular to the application of Passiflora extract in the preparation of drugs for preventing and treating breast cancer. Background technique [0002] Passionflower (Passiflora edulis), also known as "passion fruit", belongs to the Passiflora (Passiflora) plant of the Malpighiales (Malpighiales) Passifloraceae (Passifloraceae). Passiflora is native to the tropical and subtropical regions of South America and Brazil. It is introduced and cultivated all over the world because of its beautiful flowers and vine leaves that have both shade and medicinal functions. There are about more than 500 species of Passiflora in the world, of which about 50 are edible. There are more than 20 species of Passiflora in my country, mainly distributed in Guangdong, Guangxi, Fujian, Guizhou, Yunnan and Hainan. The fruit of passion fruit (P.edulis) can be used to make jam, fruit juice and red wine,...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P15/14A61P35/00A61P35/04A61K135/00A61K127/00
CPCA61K36/185A61P15/14A61P35/00A61P35/04A61K2236/333A61K2236/35A61K2236/51
Inventor 马伏宁宋顺黄东梅许奕吴斌邢文婷徐兵强郭刚王达新
Owner HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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