A 3D printing multilayer microfluidic chip and its high-throughput method for preparing liposomes
A microfluidic chip and 3D printing technology, applied in chemical instruments and methods, devices for making medicines into special physical or ingestible forms, laboratory containers, etc., can solve the problem of large injection pressure, microfluidic chip, etc. Problems such as separation and sample leakage, achieving low-cost results
Active Publication Date: 2022-06-03
CENT SOUTH UNIV
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Problems solved by technology
Currently, common microfluidic chips for liposome synthesis are difficult to achieve high-throughput liposome preparation
This is because, with the increase of the volume flux, a larger injection pressure will be generated in the microchannel, which will easily lead to the separation of the microfluidic chip and the leakage of the sample.
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[0034] The embodiments of the present invention will be described in detail below with reference to the technical solutions and the accompanying drawings.
[0035] A 3D printing multi-layer microfluidic chip and a method for high-throughput preparation of liposomes provided in this embodiment are as follows:
[0036] A 3D printed multi-layer microfluidic chip, the multi-layer micro-fluidic chip is a chip that integrates a main body 4, a multi-layer micro-channel 2, an inlet 1, an outlet 3, and a connector 5; the multi-layer micro-channel The stacks are distributed at different height positions inside the chip; the number of layers of the microchannel is 2-10; the microchannel 2 is composed of an organic solution channel 201, an organic aqueous solution channel 202, a focusing area 203, and a mixing channel 204; the The microchannel width ranges from 10 to 1000 μm, and the depth ranges from 10 to 1000 μm; the inner diameters of the inlet and the outlet range from 10 to 1500 μm;...
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Abstract
The invention provides a 3D printing multilayer microfluidic chip and a high-throughput method for preparing liposomes. The multilayer microfluidic chip produced by 3D printing is an integrated chip with multilayer microchannels, inlets, outlets and joints. The multilayer microchannels are stacked and distributed at different heights inside the chip. The microchannels are composed of organic solution channels, aqueous solution Channels, Focus Areas, Mixed Channels. Dissolve lipids and cholesterol in organic solvents according to a certain molar concentration ratio, then inject the organic solution of lipids into the organic channel of the chip, and inject the aqueous solution into the aqueous solution channel at the same time, as the organic solution and the aqueous solution are in focus When the regions converge, the concentration of the organic solvent decreases sharply, and the lipid molecules spontaneously form liposomes in water. On the one hand, the microfluidic chip avoids complex micro-nano processing technology, and has the advantages of low cost and mass production. On the other hand, its integrated multilayer microchannels enable high-throughput liposome preparation.
Description
technical field [0001] The invention belongs to the technical field of microfluidics and liposome preparation, and relates to a multi-layer microfluidic chip based on 3D printing technology and a method for preparing liposomes by using microfluidic focusing with high throughput. Background technique [0002] Liposomes are spherical vesicle structures composed of lipid bilayers. As an excellent drug carrier, liposome has high biocompatibility and biodegradability, which can increase the concentration of drugs in the body and protect the drug from being degraded. It has broad application in the fields of targeted drug delivery and molecular imaging. application prospects. At present, the commonly used methods for preparing liposomes include thin film hydration method, reverse evaporation method and ethanol / ether injection method. [0003] In 2004, Jahn et al. proposed a microfluidic focusing method for preparing liposomes using a microfluidic chip. Since the microfluidic ch...
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IPC IPC(8): B01L3/00A61J3/00
CPCB01L3/502769B01L3/502707A61J3/00B01L2300/08
Inventor 陈翔陈泽宇单晗林旗波王丹凤孙鑫全彪肖嘉莹
Owner CENT SOUTH UNIV