Brightening booster technology and brightening ampule
A technology of brightness and composition, which can be used in cosmetic preparations, medical preparations containing active ingredients, pharmaceutical formulas, etc., and can solve problems such as side effects
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Embodiment 1
[0103] (exemplary formulation)
[0104] Formulations having the ingredients disclosed herein are prepared as topical skin compositions. In some instances, topical skin compositions can be formulated as ampoules, liquids, serums, creams, gels, gels, lotions, or gel-emulsions. The formulations in Table 1 are examples of topical skin compositions prepared as ampoules.
[0105] Table 1^
[0106] Element Concentration % (by weight) water 69 PEG-8 15 Propylene Glycol 5 glycerin 4.3 Nicotinamide 3 Ethoxydiglycol 2 Phenoxyethanol 0.5 xanthan gum 0.35 plant amino acid 0.3 ferulic acid 0.25 Sodium citrate 0.2 excipient* Appropriate amount
[0107] ^ The formulation can be prepared by mixing the ingredients in a beaker with heating at 70°C to 75°C until homogeneous. The formulation can then be cooled to standard room temperature (20°C to 25°C). Also, if desired, additional ingredients can be added...
Embodiment 2
[0110] (material used)
[0111] The active ingredients in Table 2 were used to obtain the following in vitro data.
[0112] Table 2
[0113] Element supplier Nicotinamide DSM plant amino acid Carrubba ferulic acid Kinetic
Embodiment 3
[0115] (B16 Pigmentation Analysis)
[0116] Melanogenesis is the process by which melanocytes produce melanin, the naturally occurring pigment that gives skin, hair and eyes their color. Inhibiting melanogenesis is beneficial in preventing skin darkening and lightening age-related dark spots.
[0117] The B16 Pigmentation Assay utilizes the immortalized mouse melanoma cell line B16-F1 melanocytes (ATCC) to analyze the effect of the active ingredients niacinamide, plant amino acids and ferulic acid alone or in combination on melanin production. The endpoints of these assays are spectrophotometric measurements of melanin production and cellular viability.
[0118] B16-F1 melanocytes were cultured in T150 tissue culture flasks at 37 °C in 10% CO in standard DMEM growth medium supplemented with 10% fetal bovine serum (Mediatech) and 1% penicillin / streptomycin. 2 cultured until subconfluent. Cells in one confluent T150 tissue culture flask were trypsinized and resuspended in DME...
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