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Preparation method and application of chlorella extract

A chlorella extract and a chlorella technology, which are applied in the biological field, can solve the problems of unsuitable chlorella culture conditions, cumbersome extraction methods, troublesome extraction, etc., and achieve improved germination rate, simple extraction steps, and convenient extraction. Effect

Pending Publication Date: 2021-11-12
嘉兴南湖学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] During the extraction process, chlorella needs to be scientifically cultivated, but the existing chlorella culture conditions are not suitable, resulting in low extraction purity, and the extraction methods are cumbersome and troublesome

Method used

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  • Preparation method and application of chlorella extract
  • Preparation method and application of chlorella extract
  • Preparation method and application of chlorella extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A preparation method of chlorella extract comprises the following steps in sequence:

[0026] S1: Cultivation of chlorella: under sterile conditions, inoculate chlorella into BG11 medium for activation culture, and cultivate for 48-72 hours;

[0027] S2: inoculate the chlorella cultured above into the phosphorus-free BG11 medium according to the ratio of 1:5 for activation culture, culture until OD650nm=0.6, and continuously expand the algae liquid and transfer to 15L;

[0028] S3: Put the above-mentioned algae solution into a dialysis bag, centrifuge the algae solution in the dialysis bag and combine the supernatant, then use an equal volume of EA to extract, evaporate and concentrate the EA extract by a rotary evaporator until it becomes dry, and weigh the extraction The quality of the product was finally dissolved with 10mL of methanol, and stored at -4°C for later use to obtain a chlorella extract solution.

[0029] Specifically, in step S1, the specific step of cu...

Embodiment 2

[0035] Embodiment 2: Arabidopsis seed germination experiment

[0036] 1. Configuration of sample solution

[0037] Extract solution configuration:

[0038] Take by weighing phosphorus deficiency culture 7 days supernatant extract 1.1mg (according to the molecular weight 330.37 of 5-Deoxystrigol, the estimation that content is 30%) is settled in the 100mL volumetric flask with sterile water (equivalent to 10 -5 g / L), and then draw 1mL, dilute to 100mL, equivalent to 10 -7 g / L, at this time, take out 1mL and continue to configure 10 -11 g / L solution, the remaining 10 -7 The g / L extract solution is filtered through a sterile filter and placed in a container (No. 21-1) for use.

[0039] 1mL10 -7 g / L solution continued to dilute to constant volume (method is the same as 10 -7 g / L, the volume needs to be fixed twice), to get 10 -11 g / L solution, take out 1mL and continue to configure 10 -15 g / L solution, the remaining 10 -11 The g / L extract solution is filtered through a st...

Embodiment 3

[0079] Embodiment 3: Setaria seed germination experiment

[0080] 1. Pre-experimental preparation

[0081] Take an appropriate amount of purified water, put it into a 1000mL Erlenmeyer flask, pack it with a cotton plug and kraft paper, put it in a sterilizer, and sterilize it at 121°C for 20 minutes. After the end, take it out and cool it down.

[0082] Put three pieces of filter paper into petri dishes of uniform specifications, then pack the petri dishes with kraft paper, put them in a sterilizing pot, sterilize at 121°C for 20 minutes, take them out after the end, and put them in a blast drying oven. Dry the Petri dish.

[0083] Pack the small beaker and volumetric flask with kraft paper, and the Erlenmeyer flask with cotton plug and kraft paper, put them in a sterilizing pot, and sterilize at 121°C for 20 minutes. Glassware to dry.

[0084] Before each use of the ultra-clean table, wipe the table with alcohol cotton balls, put in the equipment related to the experiment,...

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Abstract

The invention discloses a preparation method of a chlorella extract. The preparation method comprises the following steps: S1, culturing chlorella; S2, inoculating the cultured chlorella into a non-phosphorus BG11 culture medium in a ratio of 1: 5, carrying out activation culture until OD650nm is 0.6, and carrying out continuous expanding culture on the chlorella solution until the volume is 15L; S3, putting the chlorella solution into a dialysis bag, centrifuging the chlorella solution in the dialysis bag, mixing supernatant liquid, performing extraction with isovolumetric EA, evaporating and concentrating an EA extracting solution by using a rotary evaporator until the EA extracting solution is dried, weighing the mass of the extract, finally dissolving out the extract by using 10mL of methanol, and performing storage at the temperature of 4 DEG C below zero for later use so as to obtain a chlorella extract solution; and S4, preparing different concentrations from the chlorella extract solution obtained in the previous step for later use. The chlorella extract is used for promoting seed germination. According to the method, simple extraction steps are adopted with convenience, and the extract has a good promoting effect on seed germination.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method and application of a chlorella extract. Background technique [0002] Chlorella sp. belongs to the genus Chlorella, Chlorococcus, Oocystaceae, and Chlorella, and is a common single-celled seaweed. Chlorella can adapt to different growth environments, and artificial cultivation is also very easy. It can not only use light energy to support itself, but also can be cultured heterotrophically. Chlorella is rich in a variety of useful components. When grown in different environments, its metabolic pathways will also change, so the accumulated products are also different. Chlorella is a good source of single-cell proteins due to its wide ecological distribution, ease of culture and rapid growth. It is a good material for biotechnology research and is rich in nutrition. Chlorella has important economic value and great application development potential. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/00C12N1/12A01C1/00C12R1/89
CPCC12P1/00C12N1/12A01C1/00
Inventor 孙诗清赵永军胡长伟刘娟徐微李俊峰曹卫星孙辰
Owner 嘉兴南湖学院
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