Modified orthopoxvirus vectors
A technology of vaccinia virus and vaccinia, which is applied in the field of immunotherapy for cell proliferation diseases, and can solve problems such as small clinical effects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment approach 191
[1859] 197. A method of treating cancer in a mammalian patient, said method comprising administering to the mammalian patient a therapeutically effective amount of the pharmaceutical composition according to embodiment 191.
[1860] 198. The method according to embodiment 196 or 197, wherein the mammalian patient is a human patient.
[1861] 199. The method according to any one of embodiments 196 to 198, wherein the cancer is selected from the group consisting of leukemia, lymphoma, liver cancer, bone cancer, lung cancer, brain cancer, bladder cancer, gastrointestinal cancer, breast cancer, cardia Cancer of the cervix, uterus, head and neck, gallbladder, larynx, lip and oral cavity, eye, melanoma, pancreas, prostate, colorectum, testis and throat.
[1862] 200. The method of embodiment 199, wherein the method further comprises administering an anti-PD1 antibody or an anti-PD-L1 antibody to the mammalian patient.
[1863] 5.7.5. Group 5
[1864] 1. A nucleic acid comprising a...
Embodiment 6-78
[2824] 6.6. Syncytia in Example 6-786-O cells
[2825] Monolayers of 786-O cells were infected with Copenhagen wild-type or CopMD5p3p virus. After 24 h, images were acquired with EVOS at 10× magnification (see Figure 10 ). This is additional evidence that syncytia occurs. exist Figure 9 In , the expression patterns of the plaques are shown. In the current experiment, monolayers of cells were infected without covering. Most of the cells infected by CopMD5p3p virus were confluent.
[2826] 6.7. Example 7 - Tumor Control and Weight Loss in a Mouse Model
[2827] Nude CD-1 mice were inoculated with HT-29 human colon carcinoma xenografts. Once a subcutaneous tumor of approximately 5 mm × 5 mm in size has formed, use 1 × 10 7 Mice were treated intravenously with PFU of either vaccinia virus three times at 24 h intervals (dashed line). Tumor size and weight loss of the mice were measured approximately every other day (see Figure 11 ). This experiment showed that CopMD5p...
Embodiment 10
[2834] 6.10. Example 10 - Immunogenicity of vaccinia in human PBMC
[2835] PBMCs were isolated from the blood of healthy human donors (n=2). PBMC were incubated with either vaccinia for 24 h and early activation markers were examined using flow cytometry (see Figure 15 ). This experiment shows that CopMD5p3p is more immunogenic and can be more easily detected by immune cells. This is considered a desirable trait because OV replication in tumor tissue requires the activation of immune cells for a successful anti-tumor immune response.
PUM
| Property | Measurement | Unit |
|---|---|---|
| length | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More