Carbon dots, preparation method thereof and application of carbon dots in preparation of mitochondria-targeting fluorescent probe

Abstract

The invention discloses a carbon dot, a preparation method thereof and application of the carbon dot in preparation of a mitochondrial targeting fluorescent probe. The preparation method of the carbon dot comprises the following steps: weighing 0.1-1g of 2,6-difluorobenzoic acid and 0.25-2.5 g of glycine for later use; adding the medicines into a reaction kettle filled with ultrapure water and absolute ethyl alcohol; heating the reaction kettle at 150-220 DEG C for 6-72 hours, and cooling the reaction kettle to room temperature; putting the obtained brown solution into ultrapure water for dialysis to remove impurities to obtain a faint yellow carbon dot solution, and performing vacuum drying to obtain carbon dot powder; then, weighing 1-30 mg of carbon dot powder and 0.1-10 g of melamine, putting into a reaction kettle filled with ultrapure water, performing constant temperature reaction at 160-220 DEG C for 1-5 hours, cooling a product to room temperature, centrifuging the product and collecting a supernatant solution, and dialyzing the supernatant solution to remove impurities, so as to obtain a modified carbon dot solution. The carbon dots prepared by the invention have high fluorescence quantum yield and stable fluorescence property, are non-toxic, and can be used as a mitochondrial fluorescence probe.

Description

technical field [0001] The invention belongs to the technical field of fluorescent carbon dots, and in particular relates to a carbon dot, a preparation method thereof and an application in preparing a fluorescent probe targeting mitochondria. Background technique [0002] Mitochondria are the powerhouses of cells. They are important organelles for maintaining cell function and metabolism. Mitochondria exhibit dynamic characteristics including fission, fusion and transport in this biological metabolic process, which can lead to mitochondrial dysfunction in certain specific environments, which can lead to the occurrence of various diseases. Therefore, it is particularly important to prepare mitochondrial imaging probes. So far, various probes have been applied to mitochondrial imaging, including fluorescent proteins, small organic molecules, and semiconductor quantum dots. But they all have some deficiencies, for example, fluorescent proteins always need high-efficiency pr...

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But its poor organelle targeting has become its biggest drawback

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