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Electrochemical method for simultaneously detecting indol sulfate and indole

A technology of indoxyl sulfate and detection method, which is applied in the field of electrochemical detection, can solve the problems of simultaneous detection of indoxyl sulfate and indole, report of electrochemical methods, etc., and avoid energy loss, low price, and time-consuming short effect

Pending Publication Date: 2021-11-26
重庆医科大学国际体外诊断研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although there have been reports on the detection of indoxyl sulfate or indole by electrochemical methods, there is no report on the simultaneous detection of indoxyl sulfate and indole.

Method used

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  • Electrochemical method for simultaneously detecting indol sulfate and indole
  • Electrochemical method for simultaneously detecting indol sulfate and indole
  • Electrochemical method for simultaneously detecting indol sulfate and indole

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Embodiment 1

[0033] The present embodiment proposes the electrochemical method of the simultaneous detection of described a kind of indoxyl sulfate and indole, specifically comprises the following steps:

[0034] 1) Using unmodified SPCE, combined with DPV method, the simultaneous detection of indoxyl sulfate and indole by electrochemical method is realized.

[0035] 2) the above-mentioned electrochemical detection method, the SPCE includes a polyethylene terephthalate (PET) substrate of a printed electrode, an outer insulating layer printed on the PET substrate and a wire interface at one end of the substrate, so There are also three electrodes printed on the above-mentioned substrate, which are carbon working electrode, Ag / AgCl reference electrode and carbon counter electrode respectively. The three electrodes form a circular working area, and each electrode passes through the wire and interface printed under the insulating film connected.

[0036] 3) The above electrochemical detection...

Embodiment 2

[0040] This embodiment is to investigate the influence of the detection solution pH value on the electrochemical behavior of indoxyl sulfate, and the detection solution is a 0.10mol / L PBS solution with a pH value of 6.0~10.0, see figure 2 . When the pH value is lower than 7.0, indoxyl sulfate produces two obvious oxidation peaks I and II (curve a and b); when the pH value is greater than 8.0, the peak current of the oxidation peak II decreases significantly (curve c ); when the pH value is greater than 9.0, the oxidation peak II approaches zero (curves d and e), indicating that the oxidation process of indoxyl sulfate is different at different pH values.

Embodiment 3

[0042] The present embodiment adopts cyclic voltammetry (CV) to investigate the electrochemical behavior of indoxyl sulfate, indole and the mixed solution of the two on SPCE, and the detection solution is 0.10mol / LpH value of 9.0 PBS solution, and the sweep speed is 50mV / s, see image 3 . Indoxyl sulfate has an obvious oxidation peak at 0.281V, no reduction peak (short horizontal line b). When indoxyl sulfate is at pH 9.0, the peak current of oxidation peak II is almost zero; There is an obvious oxidation peak at V, and there is no reduction peak (dotted line c); the mixed solution of indoxyl sulfate and indole appears two clearly separated oxidation peaks at 0.281V and 0.515V (dotted line d), The peak potential difference between the two is 234mV, indicating that the simultaneous detection of the two can be realized. It is speculated that the electrochemical reaction principle of the two can be seen in figure 1 .

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Abstract

The invention discloses an electrochemical method for simultaneously detecting indol sulfate and indole. According to the method, an unmodified screen-printed carbon electrode is taken as an electrode material, the advantages of background current reduction and detection sensitivity improvement of differential pulse voltammetry are combined, and simple, convenient and rapid simultaneous quantitative detection of indoxyl sulfate and indole is realized by adopting an electrochemical analysis technology according to a linear relationship between an electrochemical response signal and the concentration of a to-be-detected substance. The method does not depend on large-scale instruments and equipment, the unmodified silk-screen printing carbon electrode has the advantages of being easy to prepare, low in price, disposable and the like, the detection method has good repeatability and stability, and a new method is provided for studying metabolism and effects of indol sulfate and indole.

Description

technical field [0001] The invention belongs to the field of electrochemical detection, in particular to an electrochemical method for simultaneous detection of indoxyl sulfate and indole. Background technique [0002] Both indoxyl sulfate and indole are metabolites of tryptophan. Tryptophan is converted into indole by tryptophanase, and indole is metabolized into indoxyl sulfate in the liver. Indole and its derivatives are intraspecies, interspecies and transkingdom signaling molecules involved in the interaction between gut microbiota and host. In addition, indole and its derivatives may affect human diseases, such as bacterial infections, intestinal inflammation, neurological diseases and diabetes, etc. Studies have found that indole can regulate the secretion of glucagon-like peptide (GLP-1) by enteroendocrine L cells. When the body is exposed to indole for a short period of time, it will promote the secretion of GLP-1; however, when the body is exposed to indole for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/327
CPCG01N27/48G01N27/3277
Inventor 丁敏蒋梦丹张晓清朱阳李彦茹
Owner 重庆医科大学国际体外诊断研究院
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