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A kind of Bacillus veles and its application

A Bacillus, Velez technology, applied in the field of microorganisms

Active Publication Date: 2022-02-22
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limitation of bacteriostasis range and effect, it is still necessary to find strains with excellent effects for agricultural biological control

Method used

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  • A kind of Bacillus veles and its application
  • A kind of Bacillus veles and its application
  • A kind of Bacillus veles and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0034] 1. Isolation and identification of strains FB8, FB17 and FB23

[0035] (1) Isolation of strains

[0036] Strains FB8 and FB23 were isolated from jujube nectar samples in Yulin City, Shaanxi Province, and the samples were stored at room temperature after collection. Take 50mL jujube honey and heat it in a water bath at 45°C for 30 min to increase the fluidity of the honey. Take 10 mL jujube nectar and 10 mL sterile saline (preheated) and mix evenly. The diluted jujube nectar was again diluted 10 times to 10 -2 Times, 100 μL of samples diluted 2 times, 20 times and 200 times were taken respectively to coat LB medium plates, and each dilution was repeated 3 times. The plates were incubated at 37°C for 24-48h. Single colonies of different forms were picked and streaked on LB medium plates to obtain pure cultures of bacteria, and different strains were numbered, and then stored in 20% glycerol at -80°C.

[0037] Strain FB17 was isolated from honey bee samples in Xiaogan...

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Abstract

The invention discloses a bacillus velei and its application. The Bacillus Velez has been preserved in the General Microorganism Center of China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No.20022. The Bacillus Velez CGMCC No.20022 has antibacterial effect on phytopathogenic fungi, phytopathogenic bacteria, bee pathogenic fungi and bee pathogenic bacteria.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a kind of Bacillus velesii ( Bacillus velezensis ) and its applications. Background technique [0002] Bacillus ( Bacillus ) bacteria are a class of aerobic or facultative anaerobic, rod-shaped bacteria capable of producing stress-resistant endospores. Because bacillus can secrete and produce various active proteases and antibacterial active substances, it has been widely used in the fields of medicine and agriculture. Especially in the field of agriculture, bacteria of the genus Bacillus can provide a wide range of benefits to animals and plants, which can secrete a large amount of antibacterial active substances, kill some common pathogens in animals and plants, including protection of animals and plants against pathogenic microorganisms, insects and nematodes harm. In addition, Bacillus can improve the rhizosphere microbial flora, induce plant self-resistance, promote plant gr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P1/00C12R1/07
Inventor 张丽刁青云梁利王强高晶
Owner BEE RES INST CHINESE ACAD OF AGRI SCI