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Application of bacteroides fragilis and extract thereof in preparation of medicine for preventing and treating irritable bowel syndrome

A technology for irritable bowel syndrome and Bacteroides fragilis, which can be used in medicine or food, and can solve the problems that IBS cannot be completely cured and IBS lacks treatment methods.

Active Publication Date: 2021-12-03
GUANGZHOU ZHIYI PHARMA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0020] At present, there is still no very effective treatment for IBS, and IBS cannot be completely cured

Method used

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  • Application of bacteroides fragilis and extract thereof in preparation of medicine for preventing and treating irritable bowel syndrome
  • Application of bacteroides fragilis and extract thereof in preparation of medicine for preventing and treating irritable bowel syndrome
  • Application of bacteroides fragilis and extract thereof in preparation of medicine for preventing and treating irritable bowel syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Preparation Example 1 Bacteroides fragilis extract embodiment

[0076] (1) fermentation of B. fragilis

[0077] The bacteria in the blood streaked plates, anaerobic incubation 48h. Colonies were observed morphology, staining characteristics, size, shape and distribution of the club and the like.

[0078] Colony characteristics: Bacteroides fragilis ZY-312 cultured on blood plates after 48h, rendered convex circular, translucent, white, smooth surface, no hemolytic colony diameters between 1-3mm, see figure 1 .

[0079] Under microscopic morphology: Bacteroides fragilis ZY-312 Gram staining, Gram-negative bacteria, showing the typical rod, stain and blunt ends, the intermediate portion shaped like cells not stained vacuoles, see figure 2 .

[0080] Selecting a single colony was inoculated into tryptone broth fermentation is carried out for 8 hours (temperature 37 [deg.] C), the resulting precipitate was centrifuged broth speed 3000r / min, centrifuged 15min, the supernatant ...

Embodiment 2

[0089] Example 2 Effect of B. fragilis capsular polysaccharide A senna Diarrhea predominant IBS embodiment

[0090] First, experimental design

[0091] To verify Bacteroides fragilis extract provided in Example 1 of the embodiment (the main component of capsular polysaccharide A) for the prevention / treatment of irritable bowel syndrome effect, according to the present embodiment, selection of 60 C57BL / 6 mice experiment. 60 C57BL / 6 male and female mice, experimental mice were each assigned a unique number. Before the animals are grouped item number should be marked on the label squirrel, species / strain, gender, number of cages and animal numbers. BioBook using software based on the initial weight of C57BL / 6 mice were randomly divided into 6 groups, i.e., normal group (Group1 is), model group (Group2), loperamide capsule group (2.4mg / kg) (Group3), B. fragilis capsular polysaccharide A low (Group4), 10 only the (Group5), high (Group6) dose groups of C57BL / 6 mice. In thi...

Embodiment 3

[0101] Diarrhea predominant IBS Example 3 A B. fragilis capsular polysaccharide induced by castor oil.

[0102] First, experimental design

[0103] To verify Bacteroides fragilis extract provided in Example 1 of the embodiment (the main component of capsular polysaccharide A) for the prevention / treatment of irritable bowel syndrome effect, according to the present embodiment, selection of 60 C57BL / 6 mice experiment. 60 C57BL / 6 male and female mice, experimental mice were each assigned a unique number. Before the animals are grouped item number should be marked on the label squirrel, species / strain, gender, number of cages and animal numbers. BioBook using software based on the initial weight of C57BL / 6 mice were randomly divided into 6 groups, i.e., normal group (Group1 is), model group (Group2), loperamide capsule group (2.4mg / kg) (Group3), B. fragilis capsular polysaccharide A low (Group4), 10 only the (Group5), high (Group6) dose groups of C57BL / 6 mice. In this em...

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Abstract

The invention relates to application of a bacteroides fragilis extract in preparation of a medicine or food for preventing and treating irritable bowel syndrome. The bacteroides fragilis extract contains bacteroides fragilis capsular polysaccharide A. The bacteroides fragilis capsular polysaccharide A with the molecular weight of 5-70 KD is prepared accidentally, it is found accidentally that the capsular polysaccharide A with the molecular weight of 5-70 KD has a better function of preventing and treating the irritable bowel syndrome, and the effect of the capsular polysaccharide A is far better than that of the capsular polysaccharide A with the molecular weight of 110 KD extracted from bacteroides fragilis NCTC 9343.

Description

[0001] The present application is a score application for the presentation of the invention as "the application of the disconnectica extract in the preparation of drugs or foods in the preparation of intestinal pharmaceuticals". [0002] The microbial strains used in the implementation process have been preserved in the General Micro Biological Center (CGMCC) of China Microbial Square Safety Management Committee on April 2, 2015 (CGMCC) (No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing). Category Name: Smootpinetaclai ZY-312 (bacteroidesfragilis ZY-312), preservation number CGMCC No. 10685. Vulnerability pocolis ZY-312 is separated from the application unit of the present invention, and is already authorized to protect patent protection (patent number 201510459408.x), according to the provisions of the Patent Review Guide, the public can buy or authorized from commercial channels, no need to deposit, ie No need to provide a deposit certificate. Technical field [0003] Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/741A61K31/715A61K45/06A61P1/00A61P1/10A61P1/12C08B37/00A23C9/156A23C19/09A23G9/34A23K20/163
CPCA61K35/741A61P1/00A61P1/12A23C9/13A23C19/09A23G9/36A23K10/16A23K20/163A23L33/125A23L33/135A61K31/715A61K35/74A61P1/10C12N1/20Y02A50/30
Inventor 刘洋洋智发朝王晔白杨王从峰
Owner GUANGZHOU ZHIYI PHARMA INC
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