Time difference culture device

Abstract

The invention discloses a time difference culture device, and relates to the technical field of biological tissue culture. The time difference culture device is provided with a light source assembly, an observation assembly and a gas supply system; the light source assembly comprises a first light condensation part, a light attenuation piece is arranged on the light incidence side of the first light condensation part, a plurality of light transmitting parts are sequentially arranged on the light attenuation piece in the length direction of the light attenuation piece, and the light transmittance of the light transmitting parts is sequentially increased in the length direction of the light attenuation piece; the observation assembly comprises a microscopic camera and a linear driving mechanism; the gas supply system comprises a first pressure switch, a first reversing pressure release valve and a first gas storage tank, the first pressure switch is used for monitoring gas pressure in the time difference culture device and controlling the first reversing pressure release valve to act, and an outlet of the first gas storage tank is connected with an inlet of the first reversing pressure release valve after passing through a pressure reducing valve. According to the time difference culture device, the definition of a shot image can be ensured, and the image has three-dimensional sense and layering sense, so that the form of a biological tissue is accurately distinguished, and the accuracy of biological tissue evaluation analysis and screening is ensured.

Description

technical field [0001] The invention relates to the technical field of biological tissue culture, in particular to a time difference culture device. Background technique [0002] In Vitro Fertilization (In Vitro Fertilization) refers to the technology in which mammalian sperm and eggs complete the fertilization process in an artificially controlled environment outside the body. It is abbreviated as IVF in English. The existing in vitro culture of human embryos is achieved by placing the culture dish containing the embryos in a time-lag incubator or a conventional carbon dioxide incubator, while ensuring the temperature, humidity and cleanliness of the culture environment. [0003] Among them, the time difference incubator can provide a relatively closed culture and development environment for embryos, close to the mother's uterus, and can realize real-time monitoring and control adjustment of the temperature, CO2 and O2 concentration, and gas pressure of the culture environm...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the technical problem that the existing time-lag incubator adopts the illuminating lamp to observe the embryo after concentrating the light, the obtained image lacks a three-dimensional sense and a sense of layering, and it is difficult to accurately distinguish the shape of the embryo; the invention provides a time-lag culture device that can make the microscopic The image of biological tissue obtained by the camera has a sense of three-dimensionality and layering, so as to accurately identify the shape of biological tissue and ensure the accuracy of biological tissue evaluation, analysis and screening

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