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Manganese pollution repairing agent and preparation method and use thereof

A technology for pollution remediation and culture medium, applied in the field of remediation of pollution, can solve the problems of secondary damage to the environment, slow remediation, and high economic cost, and achieve the effects of environmental friendliness, simple operation, and high economic benefits.

Pending Publication Date: 2021-12-03
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of this, the application provides a manganese pollution remediation agent and its preparation method and application, which can effectively solve the existing problems in the existing abiotic manganese pollution remediation reaction, such as slow remediation, high economic cost, and easy to cause secondary damage to the environment, etc. question

Method used

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  • Manganese pollution repairing agent and preparation method and use thereof
  • Manganese pollution repairing agent and preparation method and use thereof
  • Manganese pollution repairing agent and preparation method and use thereof

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Embodiment 1

[0043] The embodiment of the present application provides a preparation method of a manganese pollution restoration agent, including:

[0044] 1) Inoculate Geobacter metallireducens and Rhodopseudomonas palustris into the culture medium of Geobacter metallireducens and Rhodopseudomonas palustris respectively at 5% (v / v) of a single bacterium, and culture at a constant temperature of 30°C.

[0045] 2) When Geobacter metallireducens and Rhodopseudomonas palustris grow to the late logarithmic phase in step 1, respectively, collect the bacterial solution by centrifugation at 7500r / min for 10min, and dilute the bacteria to OD with normal saline 600 is 0.3.

[0046] 3) Inoculate the bacterium solution in the above steps into the interoperability medium at 5% (v / v) respectively, add sodium acetate to make the final concentration 20 mM, and obtain the Geobacter-Rhodopseudomonas interoperability system.

[0047] 4) Under the constant temperature condition of 30°C, the system in step 3...

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Abstract

The invention belongs to the technical field of pollution repairing and particularly relates to a manganese pollution repairing agent and a preparation method and use thereof. On the first aspect, the manganese pollution repairing agent is prepared from geobacter metallireducens, rhodopseudomonas palustris, an organic electron donor and a syntrophic culture medium; wherein in the manganese pollution repairing agent, the geobacter metallireducens and the rhodopseudomonas palustris form a stable syntrophic relationship. The provided manganese pollution repairing agent can effectively solve problems that in an existing non-biological manganese pollution repairing reaction, repairing is slow, economic cost is high, and secondary damage to the environment is likely to be caused.

Description

technical field [0001] The application belongs to the technical field of remediation of pollution, and in particular relates to a manganese pollution remediation agent and its preparation method and application. Background technique [0002] Manganese is widely used in fields such as electrical engineering, medicine, metallurgy, chemical industry, and fertilizer. While benefiting human beings, it also causes pollution to the environment that cannot be ignored. Although a small intake of manganese can provide essential trace elements for organisms, excessive intake of manganese can cause intestinal disturbance, liver function damage, and nervous system dysfunction. [0003] In the natural environment, manganese mainly exists in the form of soluble manganese ions or insoluble manganese oxides. Among them, soluble manganese ions are bioabsorbable. Therefore, in order to reduce the biological toxicity of manganese, bioabsorbable manganese ions are usually converted into bionon...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/20
CPCC12N1/20C02F3/34B09C1/10C02F2101/206B09C2101/00
Inventor 黄玲艳袁勇周顺桂蔡茜茜刘星罗晓珊关凤仪
Owner GUANGDONG UNIV OF TECH
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