Manganese pollution repairing agent and preparation method and use thereof
A technology for pollution remediation and culture medium, applied in the field of remediation of pollution, can solve the problems of secondary damage to the environment, slow remediation, and high economic cost, and achieve the effects of environmental friendliness, simple operation, and high economic benefits.
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[0043] The embodiment of the present application provides a preparation method of a manganese pollution restoration agent, including:
[0044] 1) Inoculate Geobacter metallireducens and Rhodopseudomonas palustris into the culture medium of Geobacter metallireducens and Rhodopseudomonas palustris respectively at 5% (v / v) of a single bacterium, and culture at a constant temperature of 30°C.
[0045] 2) When Geobacter metallireducens and Rhodopseudomonas palustris grow to the late logarithmic phase in step 1, respectively, collect the bacterial solution by centrifugation at 7500r / min for 10min, and dilute the bacteria to OD with normal saline 600 is 0.3.
[0046] 3) Inoculate the bacterium solution in the above steps into the interoperability medium at 5% (v / v) respectively, add sodium acetate to make the final concentration 20 mM, and obtain the Geobacter-Rhodopseudomonas interoperability system.
[0047] 4) Under the constant temperature condition of 30°C, the system in step 3...
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