Stenotrophomonas maltophilia and application thereof

A technology of Stenotrophomonas maltophilia and maltophilia, applied in the field of Stenotrophomonas maltophilia and strains, can solve the problems of large differences in aroma substances, and achieve the effects of strong irritation, comfortable aftertaste and reduced miscellaneous gas

Pending Publication Date: 2021-12-07
CHINA TOBACCO ZHEJIANG IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The Stenotrophomonas maltophilia disclosed in the patent CN107523518B solves the problem of 2,7,11-cembratriene-4,6-diol degradation in tobacco, and proposes a specific increase in aroma substances, but it is not related to the philophilia involved in the present application. There are great differences in the aroma substances obtained by fermentation of Stenotrophomonas malt

Method used

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  • Stenotrophomonas maltophilia and application thereof
  • Stenotrophomonas maltophilia and application thereof
  • Stenotrophomonas maltophilia and application thereof

Examples

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Embodiment 1

[0032] This embodiment provides a strain of Stenotrophomonas maltophilia that can effectively enhance the aroma and remove impurities of tobacco leaves. The Stenotrophomonas maltophilia is taxonomically named Stenotrophomonas maltophilia. On April 30, 2009, it was deposited in the General Microbiology Center of China Committee for the Collection of Microbial Cultures, and its preservation number is CGMCCNO.22262; address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, postcode: 100101 , Tel: 010-64807355.

[0033] The aforementioned 16S rDNA nucleotide sequence of Stenotrophomonas maltophilia has the sequence shown in SEQ ID NO.1.

[0034] The Stenotrophomonas maltophilia strain is a Gram-positive bacterium, such as figure 1 and figure 2 As shown, the colonies on the solid medium are round, white, smooth and raised, with regular and shiny edges, which are rod-shaped bacteria.

[0035] The method for cu...

Embodiment 2

[0038] The application of a strain of Stenotrophomonas maltophilia fermented and rebaked tobacco leaves, the specific steps of the application are as follows:

[0039] 1) The formula of the medium: yeast powder 5g / L, peptone 10g / L, sodium chloride 10g / L;

[0040] 2) Strain activation: Stenotrophomonas maltophilia H-11 was activated and cultured in Example 1 to obtain Stenotrophomonas maltophilia activation liquid; the strain activation conditions were: 25-35°C, pH 5-7 , 100~150r / min shaking culture for 24~32h;

[0041] 3) Fermentation: the activated bacterium liquid obtained in step 2) is inoculated into the medium in the above-mentioned step 1) by volume ratio 1 to 5% to form a seed liquid, and the fermentation culture conditions after inoculation are the same as the activation culture conditions, and the culture time is After 12-18 hours, the seed solution was inoculated in LB medium according to the inoculation amount of 1-3%, and the culture conditions were the same as th...

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Abstract

The invention belongs to the field of bioengineering, and relates to a strain, in particular to stenotrophomonas maltophilia and application thereof. The stenotrophomonas maltophilia strain is preserved in the China General Microbiological Culture Collection Center on April 30, 2021, and the preservation number of the stenotrophomonas maltophilia strain is NO: 22262. The strain can be used as a fermentation strain to play a role in tobacco leaf fermentation after redrying. According to the stenotrophomonas maltophilia disclosed by the invention, afterfermentation with thestenotrophomonas maltophilia and redrying, aromatic substances such as 2-methyltetrahydrofuran-3-ketone, furfural, furfuryl alcohol, 4-cyclopentene-1, 3-diketone, 5-methyl-2-furfuryl alcohol, geraniol, damascone and farnesyl acetone in tobacco leaves are increased, the aroma quality and the aroma amount of the tobacco leaves are improved, irritation is reduced, and the taste of cigarettes can be improved.

Description

technical field [0001] The invention belongs to the field of bioengineering and relates to a bacterial strain, in particular to a strain of Stenotrophomonas maltophilia and its application. Background technique [0002] In the production of cigarette industry, tobacco leaves in different regions have their unique characteristics. Cigarette factories purchase tobacco leaves from tobacco farmers in various places according to their needs. However, due to the uneven quality of the purchased tobacco leaves, there are often tobacco leaves that are difficult to enter into cigarette formulas. Tobacco companies often spend a lot of money to deal with this kind of tobacco leaves. On the one hand, some tobacco leaves may be discarded due to the long storage period. This kind of tobacco leaves with less aroma, poor aroma quality, strong miscellaneous gas and high irritation are collectively referred to as unsuitable and difficult to apply tobacco leaves. The production of this kind of...

Claims

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Application Information

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IPC IPC(8): C12N1/20A24B15/20C12R1/01
CPCC12N1/20A24B15/20Y02E50/10
Inventor 李石头吴继忠帖金鑫徐清泉李永生王孟欢何文苗黄申王辉廖付张立立潘凡达田雨农毛多斌
Owner CHINA TOBACCO ZHEJIANG IND
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