Dewaxing agent for treating paraffin embedded sample and method for extracting nucleic acid from paraffin embedded sample
A technology for paraffin embedding and nucleic acid extraction, which is applied in the field of molecular biology, can solve the problems of xylene toxicity, cumbersome dewaxing process, etc., and achieve the effects of reducing toxicity, saving experimental steps and operation time, and good extraction effect
Pending Publication Date: 2021-12-07
YEASEN BIOTECHNOLOGY (SHANGHAI) CO LTD
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Problems solved by technology
At present, the most commonly used dewaxing agent for paraffin-embedded tissue is xylene, but xylene is highly toxic and the dewaxing process is cumbersome
Method used
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Experimental program
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Embodiment 1
[0040] The preparation of embodiment 1 dewaxing agent
[0041] Take 5mL of undecane and 5mL of dipentene in a 15mL centrifuge tube, shake and mix at room temperature to obtain the dewaxing agent.
Embodiment 2
[0042] The preparation of embodiment 2 dewaxing agent
[0043] Take 10mL of pristane and 10mL of 1,5-cyclooctadiene in a small beaker, stir and mix with a glass rod at room temperature to obtain a dewaxing agent.
Embodiment 3
[0044] Embodiment 3 Preparation of dewaxing agent
[0045] Take 30mL of liquid paraffin and 30mL of cycloheptene in a small beaker, stir and mix with a magnetic stirrer at room temperature to obtain a dewaxing agent.
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The invention belongs to the technical field of molecular biology, and discloses a dewaxing agent for treating a paraffin embedded sample, the dewaxing agent is a mixture of alkane and olefin, the alkane is long-chain alkane and / or cycloalkane, and the olefin is long-chain olefin and / or cycloolefin. The invention also discloses a method for extracting nucleic acid from a paraffin embedded sample. The dewaxing agent provided by the invention has the advantages of no pungent smell, small toxicity and simple dewaxing method, the steps of sample dewaxing, tissue lysis, chemical cross-linking removal and the like can be continuously carried out in the same centrifugal tube, repeated centrifugal treatment is not needed in the continuous process, and the operation steps are effectively reduced. When the dewaxing method provided by the invention is used for sample pretreatment, the DNA extracted from the sample is high in yield and good in purity, and can be used for downstream detection such as PCR, qPCR and the like.
Description
technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a dewaxing agent for processing paraffin-embedded samples and a method for extracting nucleic acid from the paraffin-embedded samples. Background technique [0002] Paraffin embedding is a common method for preserving pathological tissues in medical institutions, which are largely untapped resources and valuable resources for pathological research. However, it is difficult to extract high-quality DNA for downstream applications from these tissue samples. Extracting DNA from paraffin-embedded tissue usually requires dewaxing, digesting the tissue, removing chemical crosslinks, eluting impurity proteins and salt ions, and finally obtaining purified DNA. Currently, the most commonly used dewaxing agent for paraffin-embedded tissues is xylene, but xylene is highly toxic and the dewaxing process is cumbersome. Therefore, there is an urgent need for a dewaxing a...
Claims
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IPC IPC(8): C12N15/10
CPCC12N15/1013C12Q2523/308C12Q2523/32C12Q2521/537
Inventor 宋东亮李明孙晓亮
Owner YEASEN BIOTECHNOLOGY (SHANGHAI) CO LTD